WFRF:(Meisel H)
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| Fältnamn | Indikatorer | Metadata |
|---|---|---|
| 000 | 03269naa a2200409 4500 | |
| 001 | oai:prod.swepub.kib.ki.se:1927869 | |
| 003 | SwePub | |
| 008 | 240701s2002 | |||||||||||000 ||eng| | |
| 024 | 7 | a http://kipublications.ki.se/Default.aspx?queryparsed=id:19278692 URI |
| 024 | 7 | a https://doi.org/10.1159/0000679272 DOI |
| 040 | a (SwePub)ki | |
| 041 | a engb eng | |
| 042 | 9 SwePub | |
| 072 | 7 | a ref2 swepub-contenttype |
| 072 | 7 | a art2 swepub-publicationtype |
| 100 | 1 | a Kazaks, A4 aut |
| 245 | 1 0 | a Stop codon insertion restores the particle formation ability of hepatitis B virus core-hantavirus nucleocapsid protein fusions |
| 264 | c 2003-01-30 | |
| 264 | 1 | b S. Karger AG,c 2002 |
| 520 | a In recent years, epitopes of various origin have been inserted into the core protein of hepatitis B virus (HBc), allowing the formation of chimeric HBc particles. Although the C-terminus of a C-terminally truncated HBc (HBcΔ) tolerates the insertion of extended foreign sequences, the insertion capacity is still a limiting factor for the construction of multivalent vaccines. Previously, we described a new system to generate HBcΔ mosaic particles based on a read-through mechanism in an <i>Escherichia coli</i> suppressor strain [J Gen Virol 1997;78:2049–2053]. Those mosaic particles allowed the insertion of a 114-amino acid (aa)-long segment of a Puumala hantavirus (PUUV) nucleocapsid (N) protein. To study the value and the potential limitations of the mosaic approach in more detail, we investigated the assembly capacity of ‘non-mosaic’ HBcΔ fusion proteins and the corresponding mosaic constructs carrying 94, 213 and 433 aa of the hantaviral N protein. Whereas the fusion proteins carrying 94, 114, 213 or 433 aa were not assembled into HBcΔ particles, or only at a low yield, the insertion of a stop codon-bearing linker restored the ability to form particles with 94, 114 and 213 foreign aa. The mosaic particles formed exhibited PUUV-N protein antigenicity. Immunization of BALB/c mice with these mosaic particles carrying PUUV-N protein aa 1–114, aa 1–213 and aa 340–433, respectively, induced HBc-specific antibodies, whereas PUUV-N protein-specific antibodies were detected only in mice immunized with particles carrying N-terminal aa 1–114 or aa 1–213 of the N protein. Both the anti-HBc and anti-PUUV antibody responses were IgG1 dominated. In conclusion, stop codon suppression allows the formation of mosaic core particles carrying large-sized and ‘problematic’, e.g. hydrophobic, hantavirus sequences. | |
| 700 | 1 | a Lachmann, S4 aut |
| 700 | 1 | a Koletzki, D4 aut |
| 700 | 1 | a Petrovskis, I4 aut |
| 700 | 1 | a Dislers, A4 aut |
| 700 | 1 | a Ose, V4 aut |
| 700 | 1 | a Skrastina, D4 aut |
| 700 | 1 | a Gelderblom, HR4 aut |
| 700 | 1 | a Lundkvist, Au Karolinska Institutet4 aut |
| 700 | 1 | a Meisel, H4 aut |
| 700 | 1 | a Borisova, G4 aut |
| 700 | 1 | a Kruger, DH4 aut |
| 700 | 1 | a Pumpens, P4 aut |
| 700 | 1 | a Ulrich, R4 aut |
| 710 | 2 | a Karolinska Institutet4 org |
| 773 | 0 | t Intervirologyd : S. Karger AGg 45:4-6, s. 340-349q 45:4-6<340-349x 0300-5526x 1423-0100 |
| 856 | 4 8 | u http://kipublications.ki.se/Default.aspx?queryparsed=id:1927869 |
| 856 | 4 8 | u https://doi.org/10.1159/000067927 |
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- Kazaks, A
- Lachmann, S
- Koletzki, D
- Petrovskis, I
- Dislers, A
- Ose, V
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- Skrastina, D
- Gelderblom, HR
- Lundkvist, A
- Meisel, H
- Borisova, G
- Kruger, DH
- Pumpens, P
- Ulrich, R
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- Intervirology
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- Karolinska Institutet
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