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Determination of 21...
Determination of 21-hydroxylase autoantibodies: inter-laboratory concordance in the Euradrenal International Serum Exchange Program
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- Falorni, Alberto (författare)
- Univ Perugia, Dept Med, I-06126 Perugia, Italy.
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- Bini, Vittorio (författare)
- Univ Perugia, Dept Med, I-06126 Perugia, Italy.
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- Betterle, Corrado (författare)
- Univ Padua, Dept Med, Endocrine Unit, Padua, Italy.
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- Brozzetti, Annalisa (författare)
- Univ Perugia, Dept Med, I-06126 Perugia, Italy.
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- Castano, Luis (författare)
- Univ Basque Country, Cruces Univ Hosp, Ciberdem, BioCruces, Bilbao, Spain.
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- Fichna, Marta (författare)
- Poznan Univ Med Sci, Dept Endocrinol & Metab, Poznan, Poland.;Polish Acad Sci, Inst Human Genet, PL-60479 Poznan, Poland.
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- Kämpe, Olle (författare)
- Karolinska Institutet,Uppsala universitet,Autoimmunitet,Science for Life Laboratory, SciLifeLab
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- Mellgren, Gunnar (författare)
- Haukeland Hosp, Hormone Lab, N-5021 Bergen, Norway.;Univ Bergen, Dept Clin Sci, Bergen, Norway.
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- Peterson, Part (författare)
- Univ Tartu, Inst Biomed & Translat Med, Mol Pathol, EE-50090 Tartu, Estonia.
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- Chen, Shu (författare)
- RSR Ltd, FIRS Labs, Cardiff, S Glam, Wales.
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- Rönnelid, Johan (författare)
- Uppsala universitet,Klinisk immunologi
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- Seissler, Jochen (författare)
- Klinikum Univ Munchen, Med Klin & Poliklin 4, Diabet Zentrum, Munich, Germany.
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- Tiberti, Claudio (författare)
- Univ Roma La Sapienza, Dept Expt Med, I-00185 Rome, Italy.
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- Uibo, Raivo (författare)
- Univ Tartu, Inst Biomed & Translat Med, Dept Immunol, EE-50090 Tartu, Estonia.
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- Yu, Liping (författare)
- Univ Colorado Denver, Barbara Davis Ctr Diabet, Aurora, CO USA.
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- Lernmark, Åke (författare)
- Lund University,Lunds universitet,Celiaki och diabetes,Forskargrupper vid Lunds universitet,Celiac Disease and Diabetes Unit,Lund University Research Groups,Lund Univ, Skane Univ Hosp, Dept Clin Sci, Malmo, Sweden.
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- Husebye, Eystein (författare)
- Univ Bergen, Dept Clin Sci, Bergen, Norway.;Haukeland Hosp, Dept Med, N-5021 Bergen, Norway.
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Univ Perugia, Dept Med, I-06126 Perugia, Italy Univ Padua, Dept Med, Endocrine Unit, Padua, Italy. (creator_code:org_t)
- Walter de Gruyter GmbH, 2015
- 2015
- Engelska.
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Ingår i: Clinical Chemistry and Laboratory Medicine. - : Walter de Gruyter GmbH. - 1434-6621 .- 1437-4331. ; 53:11, s. 1761-1770
- Relaterad länk:
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http://dx.doi.org/10...
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https://lup.lub.lu.s...
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https://doi.org/10.1...
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https://urn.kb.se/re...
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http://kipublication...
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Abstract
Ämnesord
Stäng
- Background: 21-Hydroxylase autoantibodies (21OHAb) are markers of an adrenal autoimmune process that identifies individuals with autoimmune Addison's disease (AAD). Quality and inter-laboratory agreement of various 21OHAb tests are incompletely known. The objective of the study was to determine inter-laboratory concordance for 21OHAb determinations. Methods: Sixty-nine sera from 51 patients with AAD and 51 sera from 51 healthy subjects were blindly coded by a randomization center and distributed to 14 laboratories that determined 21OHAb, either by an "in-house" assay (n=9) using in vitro-translated S-35-21OH or luciferase-labeled 21OH or a commercial kit with I-125-21OH (n=5). Main outcome measures were diagnostic accuracy of each participating laboratory and inter-laboratory agreement of 21OHAb assays. Results: Intra-assay coefficient of variation ranged from 2.6% to 5.3% for laboratories using the commercial kit and from 5.1% to 23% for laboratories using "in-house" assays. Diagnostic accuracy, expressed as area under ROC curve (AUC), varied from 0.625 to 0.947 with the commercial kit and from 0.562 to 0.978 with "in-house" methods. Cohen's. of inter-rater agreement was 0.603 among all 14 laboratories, 0.691 among "in-house" laboratories, and 0.502 among commercial kit users. Optimized cutoff levels, calculated on the basis of AUCs, increased the diagnostic accuracy of every laboratory (AUC >0.9 for 11/14 laboratories) and increased the Cohen's. of inter-rater agreement. Discrepancies in quantitation of 21OHAb levels among different laboratories increased with increasing autoantibody levels. Conclusions: The quality of 21OHAb analytical procedures is mainly influenced by selection of cutoff value and correct handling of assay materials. A standardization program is needed to identify common standard sera and common measuring units.
Ämnesord
- MEDICIN OCH HÄLSOVETENSKAP -- Klinisk medicin -- Reumatologi och inflammation (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Clinical Medicine -- Rheumatology and Autoimmunity (hsv//eng)
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Immunologi inom det medicinska området (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Immunology in the medical area (hsv//eng)
Nyckelord
- Addison's disease
- adrenal antibodies
- autoimmunity
- diagnosis
- RIA
- standardization
- Addison's disease
Publikations- och innehållstyp
- art (ämneskategori)
- ref (ämneskategori)
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Falorni, Alberto
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Bini, Vittorio
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Betterle, Corrad ...
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Brozzetti, Annal ...
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Castano, Luis
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Fichna, Marta
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visa fler...
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Kämpe, Olle
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Mellgren, Gunnar
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Peterson, Part
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Chen, Shu
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Rönnelid, Johan
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Seissler, Jochen
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Tiberti, Claudio
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Uibo, Raivo
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Yu, Liping
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Lernmark, Åke
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Husebye, Eystein
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Lunds universitet
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Uppsala universitet
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Karolinska Institutet