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Reproducible diagno...
Reproducible diagnosis of chronic lymphocytic leukemia by flow cytometry : An European Research Initiative on CLL (ERIC) & European Society for Clinical Cell Analysis (ESCCA) Harmonisation project
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- Rawstron, Andy C. (author)
- St Jamess Inst Oncol, Leeds
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- Kreuzer, Karl-Anton (author)
- Universitätsklinikum Köln, Köln
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- Soosapilla, Asha (author)
- Laverty Pathol, Sydney
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- Spacek, Martin (author)
- Univ Hosp, Prague
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- Stehlikova, Olga (author)
- Masaryk Univ, CEITEC, Brno; Univ Hosp Brno, Dept Internal Med Hematol & Oncol, Brno
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- Gambell, Peter (author)
- Peter MacCallum Canc Ctr, Melbourne
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- McIver-Brown, Neil (author)
- Royal Bournemouth Hosp, Bournemouth
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- Villamor, Neus (author)
- Hosp Clin Barcelona, Hematopathol Unit, Barcelona
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- Psarra, Katherina (author)
- Evangelismos Med Ctr, Athens
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- Arroz, Maria (author)
- CHLO Hosp S Francisco Xavier, Dept Clin Pathol, Flow Cytometry Lab, Lisbon
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- Milani, Raffaella (author)
- Osped San Raffaele, Milan
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- de la Serna, Javier (author)
- Hosp Univ 12 Octubre, Madrid
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- Teresa Cedena, M. (author)
- Hosp Univ 12 Octubre, Madrid
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- Jaksic, Ozren (author)
- Dubrava Univ Hosp, Zagreb
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- Nomdedeu, Josep (author)
- Hosp Santa Creu & Sant Pau, Barcelona
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- Moreno, Carol (author)
- Hosp Santa Creu & Sant Pau, Barcelona
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- Rigolin, Gian Matteo (author)
- St Anna Univ Hosp, Ferrara
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- Cuneo, Antonio (author)
- St Anna Univ Hosp, Ferrara
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- Johansen, Preben (author)
- Aalborg Univ Hosp, Dept Haematol, Clin Canc Res Ctr, Aalborg; Aalborg Univ, Dept Clin Med, Aalborg
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- Johnsen, Hans E. (author)
- Aalborg Univ Hosp, Dept Haematol, Clin Canc Res Ctr, Aalborg; Aalborg Univ, Dept Clin Med, Aalborg
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- Rosenquist, Richard (author)
- Karolinska Institutet,Uppsala universitet,Experimentell och klinisk onkologi
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- Niemann, Carsten Utoft (author)
- Copenhagen Univ Hosp, Rigshosp, Copenhagen
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- Kern, Wolfgang (author)
- MLL Munich Leukemia Lab, Munich
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- Westerman, David (author)
- Univ Hosp Brno, Dept Internal Med Hematol & Oncol, Brno
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- Trneny, Marek (author)
- Univ Hosp, Prague
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- Mulligan, Stephen (author)
- Laverty Pathol, Sydney
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- Doubek, Michael (author)
- Masaryk Univ, CEITEC, Brno
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- Pospisilova, Sarka (author)
- Masaryk Univ, CEITEC, Brno
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- Hillmen, Peter (author)
- St Jamess Inst Oncol, Leeds
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- Oscier, David (author)
- Royal Bournemouth Hosp, Bournemouth
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- Hallek, Michael (author)
- Univ Klinikum Köln, Köln
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- Ghia, Paolo (author)
- Univ Vita Salute San Raffaele, Milan; IRCCS Ist Sci San Raffaele, Milan
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- Montserrat, Emili (author)
- Univ Barcelona, Hosp Clin, Dept Hematol, Barcelona
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(creator_code:org_t)
- 2018-01-17
- 2018
- English.
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In: Cytometry. Part B, Clinical cytometry.. - : Wiley. - 1552-4949 .- 1552-4957. ; 94:1, s. 121-128
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Abstract
Subject headings
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- The diagnostic criteria for CLL rely on morphology and immunophenotype. Current approaches have limitations affecting reproducibility and there is no consensus on the role of new markers. The aim of this project was to identify reproducible criteria and consensus on markers recommended for the diagnosis of CLL. ERIC/ESCCA members classified 14 of 35 potential markers as “required” or “recommended” for CLL diagnosis, consensus being defined as >75% and >50% agreement, respectively. An approach to validate “required” markers using normal peripheral blood was developed. Responses were received from 150 participants with a diagnostic workload >20 CLL cases per week in 23/150 (15%), 5–20 in 82/150 (55%), and <5 cases per week in 45/150 (30%). The consensus for “required” diagnostic markers included: CD19, CD5, CD20, CD23, Kappa, and Lambda. “Recommended” markers potentially useful for differential diagnosis were: CD43, CD79b, CD81, CD200, CD10, and ROR1. Reproducible criteria for component reagents were assessed retrospectively in 14,643 cases from 13 different centers and showed >97% concordance with current approaches. A pilot study to validate staining quality was completed in 11 centers. Markers considered as “required” for the diagnosis of CLL by the participants in this study (CD19, CD5, CD20, CD23, Kappa, and Lambda) are consistent with current diagnostic criteria and practice. Importantly, a reproducible approach to validate and apply these markers in individual laboratories has been identified. Finally, a consensus “recommended” panel of markers to refine diagnosis in borderline cases (CD43, CD79b, CD81, CD200, CD10, and ROR1) has been defined and will be prospectively evaluated.
Subject headings
- MEDICIN OCH HÄLSOVETENSKAP -- Klinisk medicin -- Klinisk laboratoriemedicin (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Clinical Medicine -- Clinical Laboratory Medicine (hsv//eng)
Keyword
- chronic lymphocytic leukemia
- flow cytometry
- diagnosis
Publication and Content Type
- ref (subject category)
- art (subject category)
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- By the author/editor
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Rawstron, Andy C ...
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Kreuzer, Karl-An ...
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Soosapilla, Asha
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Spacek, Martin
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Stehlikova, Olga
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Gambell, Peter
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show more...
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McIver-Brown, Ne ...
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Villamor, Neus
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Psarra, Katherin ...
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Arroz, Maria
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Milani, Raffaell ...
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de la Serna, Jav ...
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Teresa Cedena, M ...
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Jaksic, Ozren
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Nomdedeu, Josep
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Moreno, Carol
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Rigolin, Gian Ma ...
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Cuneo, Antonio
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Johansen, Preben
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Johnsen, Hans E.
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Rosenquist, Rich ...
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Niemann, Carsten ...
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Kern, Wolfgang
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Westerman, David
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Trneny, Marek
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Mulligan, Stephe ...
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Doubek, Michael
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Pospisilova, Sar ...
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Hillmen, Peter
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Oscier, David
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Hallek, Michael
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Ghia, Paolo
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Montserrat, Emil ...
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- About the subject
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- MEDICAL AND HEALTH SCIENCES
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MEDICAL AND HEAL ...
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and Clinical Medicin ...
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and Clinical Laborat ...
- Articles in the publication
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Cytometry. Part ...
- By the university
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Uppsala University
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Karolinska Institutet