Screening for Differentially Expressed Circular RNAs in the Cartilage of Osteoarthritis Patients for Their Diagnostic Value

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Fältnamn	Indikatorer	Metadata
000		06477naa a2200589 4500
001		oai:DiVA.org:umu-163635
003		SwePub
008		191001s2019 \| \|\|\|\|\|\|\|\|\|\|\|000 \|\|eng\|
024	7	a https://urn.kb.se/resolve?urn=urn:nbn:se:umu:diva-1636352 URI
024	7	a https://doi.org/10.1089/gtmb.2019.01082 DOI
040		a (SwePub)umu
041		a engb eng
042		9 SwePub
072	7	a ref2 swepub-contenttype
072	7	a art2 swepub-publicationtype
100	1	a Wang, Yingu Department of Orthopedics, the First Affiliated Hospital of Xi'an Jiao Tong University, Xi'an, People's Republic of China4 aut
245	1 0	a Screening for Differentially Expressed Circular RNAs in the Cartilage of Osteoarthritis Patients for Their Diagnostic Value
264	1	b Mary Ann Liebert,c 2019
338		a print2 rdacarrier
520		a Background: Osteoarthritis (OA) is the most prevalent osteoarticular disease, which typically involves chronic cartilage degeneration and synovitis. The latest research shows that circular RNAs (circRNAs) play a role in the development of a variety of diseases, including osteoarthrosis.Purposes: The aim of this study was to explore the expression of circRNAs in OA chondrocytes and predict biomarkers for diagnosis.Materials and Methods: The circRNA expression profile was analyzed through use of the Gene Spring software V13.0; differentially expressed circRNAs were screened by comparing OA chondrocytes and normal articular chondrocytes. We validated the microarray data by quantitative real-time polymerase chain reaction analyses of OA chondrocytes and chondrocytes from normal controls. TargetScan software and miRanda software were used to predict networks of circRNA–miRNA interactions in cartilage. Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway and Gene Ontology (GO) analyses were applied to predict the functions of differentially expressed circRNAs.Results: Overall, 1380 circRNAs were differentially expressed between OA chondrocytes and normal articular chondrocytes (fold-change ≥2, p ≤ 0.05), including 215 that were upregulated and 1165 that were downregulated circRNAs. After analyzing the differentially expressed circRNA genes, the top 20 enriched GO entries and KEGG pathways were annotated. The hsa_circrna_0032131 was identified for further analysis. A circRNA–miRNA network was constructed to represent the 10 most likely target genes associated with the validated circRNA.Conclusions: Our research suggests that some of the differentially expressed circRNAs in OA chondrocytes compared to normal chondrocytes are etiologically associated with the pathological process of OA. It was found that hsa_circRNA_0032131 likely participates in the initiation and progression of OA and has potential as a diagnostic marker.Clinical Relevance: To analyze the difference of circRNA expression profiles between OA and normal controls and explore biomarkers for diagnosis.
650	7	a MEDICIN OCH HÄLSOVETENSKAPx Medicinska och farmaceutiska grundvetenskaperx Cell- och molekylärbiologi0 (SwePub)301082 hsv//swe
650	7	a MEDICAL AND HEALTH SCIENCESx Basic Medicinex Cell and Molecular Biology0 (SwePub)301082 hsv//eng
650	7	a MEDICIN OCH HÄLSOVETENSKAPx Klinisk medicinx Ortopedi0 (SwePub)302112 hsv//swe
650	7	a MEDICAL AND HEALTH SCIENCESx Clinical Medicinex Orthopaedics0 (SwePub)302112 hsv//eng
650	7	a MEDICIN OCH HÄLSOVETENSKAPx Klinisk medicinx Reumatologi och inflammation0 (SwePub)302102 hsv//swe
650	7	a MEDICAL AND HEALTH SCIENCESx Clinical Medicinex Rheumatology and Autoimmunity0 (SwePub)302102 hsv//eng
650	7	a MEDICIN OCH HÄLSOVETENSKAPx Medicinsk bioteknologix Biomedicinsk laboratorievetenskap/teknologi0 (SwePub)304022 hsv//swe
650	7	a MEDICAL AND HEALTH SCIENCESx Medical Biotechnologyx Biomedical Laboratory Science/Technology0 (SwePub)304022 hsv//eng
653		a osteoarthritis
653		a circRNAs
653		a expression profile
653		a functional analysis
653		a molekylärbiologi
653		a Molecular Biology
653		a Clinical Chemistry
653		a klinisk kemi
653		a Orthopaedics
653		a ortopedi
653		a reumatologi
653		a rheumatology
653		a medicinsk biokemi
653		a Medical Biochemistry
700	1	a Wu, Cuiyanu School of Public Health, Xi'an Jiaotong University Health Science Center, Key Laboratory of Trace Elements and Endemic Diseases, National Health and Family Planning Commission, Xi'an, People's Republic of China4 aut
700	1	a Zhang, Fengu School of Public Health, Xi'an Jiaotong University Health Science Center, Key Laboratory of Trace Elements and Endemic Diseases, National Health and Family Planning Commission, Xi'an, People's Republic of China4 aut
700	1	a Zhang, Yananu School of Public Health, Xi'an Jiaotong University Health Science Center, Key Laboratory of Trace Elements and Endemic Diseases, National Health and Family Planning Commission, Xi'an, People's Republic of China4 aut
700	1	a Ren, Zhiweiu Department of Orthopedics, the First Affiliated Hospital of Xi'an Jiao Tong University, Xi'an, People's Republic of China4 aut
700	1	a Lammi, Mikko J.,d 1961-u Umeå universitet,Institutionen för integrativ medicinsk biologi (IMB),School of Public Health, Xi'an Jiaotong University Health Science Center; Key Laboratory of Trace Elements and Endemic Diseases, National Health and Family Planning Commission, Xi'an, People's Republic of China,Chondrogen ic and Osteogenic Differentiation4 aut0 (Swepub:umu)mila0077
700	1	a Guo, Xiongu School of Public Health, Xi'an Jiaotong University Health Science Center, Key Laboratory of Trace Elements and Endemic Diseases, National Health and Family Planning Commission, Xi'an, People's Republic of China4 aut
710	2	a Department of Orthopedics, the First Affiliated Hospital of Xi'an Jiao Tong University, Xi'an, People's Republic of Chinab School of Public Health, Xi'an Jiaotong University Health Science Center, Key Laboratory of Trace Elements and Endemic Diseases, National Health and Family Planning Commission, Xi'an, People's Republic of China4 org
773	0	t Genetic Testing and Molecular Biomarkersd : Mary Ann Liebertg 23:10, s. 706-716q 23:10<706-716x 1945-0265x 1945-0257
856	4 8	u https://urn.kb.se/resolve?urn=urn:nbn:se:umu:diva-163635
856	4 8	u https://doi.org/10.1089/gtmb.2019.0108

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Av författaren/redakt...: Wang, Ying; Wu, Cuiyan; Zhang, Feng; Zhang, Yanan; Ren, Zhiwei; Lammi, Mikko J., ...; visa fler...; Guo, Xiong; visa färre...

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