WFRF:(Zhang Xue Qiang)
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User-Friendly Genetic Conditional Knockout Strategies by CRISPR/Cas9
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- Chen, Liangliang (author)
- Soochow Univ, Cam Su Genom Resource Ctr, Suzhou 215123, Peoples R China.
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- Ye, Ying (author)
- Soochow Univ, Cam Su Genom Resource Ctr, Suzhou 215123, Peoples R China.
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- Dai, Hongxia (author)
- Soochow Univ, Cam Su Genom Resource Ctr, Suzhou 215123, Peoples R China.
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- Soochow Univ, Cam Su Genom Resource Ctr, Suzhou 215123, Peoples R China Macau Univ Sci & Technol, State Key Lab Qual Res Chinese Med, Ave Wai Long, Taipa, Macau, Peoples R China. (creator_code:org_t)
- HINDAWI LTD, 2018
- 2018
- English.
- In: STEM CELLS INTERNATIONAL. - : HINDAWI LTD. - 1687-966X .- 1687-9678.
- Journal article (peer-reviewed)
Abstract
Subject headings
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- Loss-of-function studies are critically important in gene functional analysis of model organisms and cells. However, conditional gene inactivation in diploid cells is difficult to achieve, as it involves laborious vector construction, multifold electroporation, and complicated genotyping. Here, a strategy is presented for generating biallelic conditional gene and DNA regulatory region knockouts in mouse embryonic stem cells by codelivery of CRISPR-Cas9 and short-homology-arm targeting vectors sequentially or simultaneously. Collectively, a simple and rapid method was presented to knock out any DNA element conditionally. This approach will facilitate the functional studies of essential genes and regulatory regions during development.
Subject headings
- NATURVETENSKAP -- Biologi -- Utvecklingsbiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Developmental Biology (hsv//eng)
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- ref (subject category)
- art (subject category)
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- Chen, Liangliang
- Ye, Ying
- Dai, Hongxia
- Zhang, Heyao
- Zhang, Xue
- Wu, Qiang
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- Zhu, Zhexin
- Spalinskas, Rapo ...
- Ren, Wenyan
- Zhang, Wensheng
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- NATURAL SCIENCES
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- Royal Institute of Technology
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