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Characterization of...
Characterization of Chlamydia trachomatis omp1 genotypes among sexually transmitted disease patients in Sweden
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- Jurstrand, Margaretha (författare)
- Örebro universitet,Institutionen för klinisk medicin,Department of Clinical Microbiology and Immunology, Örebro Medical Centre Hospital, Örebro
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- Falk, Lars (författare)
- Outpatient Sexually Transmitted Disease Clinic, Department of Dermatovenereology, Örebro Medical Centre Hospital, Örebro
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- Fredlund, Hans (författare)
- Region Örebro län,Department of Clinical Microbiology and Immunology, Örebro Medical Centre Hospital, Örebro
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- Lindberg, Margareta (författare)
- Outpatient Sexually Transmitted Disease Clinic, Department of Dermatovenereology, Örebro Medical Centre Hospital, Örebro
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- Olcén, Per, 1943- (författare)
- Department of Clinical Microbiology and Immunology, Örebro Medical Centre Hospital, Örebro
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- Andersson, Sören (författare)
- Department of Clinical Microbiology and Immunology, Örebro Medical Centre Hospital, Örebro
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- Persson, Kenneth (författare)
- Lund University,Lunds universitet,Klinisk mikrobiologi, Malmö,Forskargrupper vid Lunds universitet,Clinical Microbiology, Malmö,Lund University Research Groups,Department of Clinical Microbiology, Malmö University Hospital, Malmö
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- Albert, Jan (författare)
- Karolinska Institutet,Division of Virology, Department of Microbiology, Pathology and Immunology, Karolinska Institutet, Huddinge University Hospital, Huddinge/Stockholm, Sweden
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- Bäckman, Anders (författare)
- Region Örebro län,Department of Clinical Microbiology and Immunology, Örebro Medical Centre Hospital, Örebro
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(creator_code:org_t)
- 2001
- 2001
- Engelska.
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Ingår i: Journal of Clinical Microbiology. - 0095-1137 .- 1098-660X. ; 39:11, s. 3915-3919
- Relaterad länk:
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http://dx.doi.org/10...
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https://urn.kb.se/re...
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https://doi.org/10.1...
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http://kipublication...
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https://lup.lub.lu.s...
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https://urn.kb.se/re...
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Abstract
Ämnesord
Stäng
- A method for detection and genotyping of genital Chlamydia trachomatis infections based on omp1 gene amplification and sequencing was developed. DNA was extracted from urogenital or urine samples using a Chelex-based method, and an approximately 1,100-bp-long fragment from the omp1 gene was directly amplified and sequenced. Genotyping was performed by BLAST similarity search, and phylogenetic tree analysis was used to illustrate the evolutionary relationships between clinical isolates and reference strains. The method was used to determine the genotypes of C. trachomatis in 237 positive urogenital and/or urine specimens collected at a Swedish sexually transmitted disease clinic during 1 year. The most common genotypes corresponded to serotypes E (47%) and F (17%). The omp1 gene was highly conserved for genotype E (106 of 112 samples without any mutation) and F (41 of 42 samples without any mutation) strains but appear slightly less conserved for genotypes G (n = 6) and H (n = 6), where the sequences displayed one to four nucleotide substitutions relative to the reference sequence. Genotyping of samples collected at the follow-up visit indicated that two patients had become reinfected, while three other patients suffered treatment failure or reinfection. One woman appeared to have a mixed infection with two different C. trachomatis strains. This omp1 genotyping method had a high reproducibility and could be used for epidemiological characterization of sexually transmitted Chlamydia infections.
Ämnesord
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Mikrobiologi inom det medicinska området (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Microbiology in the medical area (hsv//eng)
Nyckelord
- MEDICINE
- MEDICIN
- Medicine
- Medicin
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
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- Av författaren/redakt...
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Jurstrand, Marga ...
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Falk, Lars
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Fredlund, Hans
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Lindberg, Margar ...
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Olcén, Per, 1943 ...
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Andersson, Sören
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visa fler...
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Persson, Kenneth
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Albert, Jan
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Bäckman, Anders
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Örebro universitet
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Karolinska Institutet
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Lunds universitet
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