(WFRF:(Westerlund Anna 1978 ))
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| Fältnamn | Indikatorer | Metadata |
|---|---|---|
| 000 | 03940naa a2200505 4500 | |
| 001 | oai:research.chalmers.se:fb16d2c8-136d-4f98-b4e4-8e3931fe2d49 | |
| 003 | SwePub | |
| 008 | 210827s2021 | |||||||||||000 ||eng| | |
| 024 | 7 | a https://research.chalmers.se/publication/5257312 URI |
| 024 | 7 | a https://doi.org/10.3390/ijms221690082 DOI |
| 040 | a (SwePub)cth | |
| 041 | a engb eng | |
| 042 | 9 SwePub | |
| 072 | 7 | a art2 swepub-publicationtype |
| 072 | 7 | a ref2 swepub-contenttype |
| 100 | 1 | a Wypijewska Del Nogal, Anna Wiktoria,d 1982u Chalmers tekniska högskola,Chalmers University of Technology4 aut0 (Swepub:cth)nogal |
| 245 | 1 0 | a Complex conformational dynamics of the heart failure‐associated pre‐mirna‐377 hairpin revealed by single‐molecule optical tweezers |
| 264 | c 2021-08-20 | |
| 264 | 1 | b MDPI AG,c 2021 |
| 338 | a electronic2 rdacarrier | |
| 520 | a pre‐miRNA‐377 is a hairpin‐shaped regulatory RNA associated with heart failure. Here, we use single‐molecule optical tweezers to unzip pre‐miRNA‐377 and study its stability and dy-namics. We show that magnesium ions have a strong stabilizing effect, and that sodium ions stabi-lize the hairpin more than potassium ions. The hairpin unfolds in a single step, regardless of buffer composition. Interestingly, hairpin folding occurs either in a single step (type 1) or through the formation of intermediates, in multiple steps (type 2) or gradually (type 3). Type 3 occurs only in the presence of both sodium and magnesium, while type 1 and 2 take place in all buffers, with type 1 being the most prevalent. By reducing the size of the native hairpin loop from fourteen to four nu-cleotides, we demonstrate that the folding heterogeneity originates from the large size of the hairpin loop. Further, while efficient pre‐miRNA‐377 binders are lacking, we demonstrate that the recently developed C2 ligand displays bimodal activity: it enhances the mechanical stability of the pre-miRNA‐377 hairpin and perturbs its folding. The knowledge regarding pre‐miRNA stability and dynamics that we provide is important in understanding its regulatory function and how it can be modulated to achieve a therapeutic effect, e.g., in heart failure treatment. | |
| 650 | 7 | a NATURVETENSKAPx Biologix Biokemi och molekylärbiologi0 (SwePub)106022 hsv//swe |
| 650 | 7 | a NATURAL SCIENCESx Biological Sciencesx Biochemistry and Molecular Biology0 (SwePub)106022 hsv//eng |
| 650 | 7 | a NATURVETENSKAPx Kemix Materialkemi0 (SwePub)104032 hsv//swe |
| 650 | 7 | a NATURAL SCIENCESx Chemical Sciencesx Materials Chemistry0 (SwePub)104032 hsv//eng |
| 650 | 7 | a NATURVETENSKAPx Biologix Biofysik0 (SwePub)106032 hsv//swe |
| 650 | 7 | a NATURAL SCIENCESx Biological Sciencesx Biophysics0 (SwePub)106032 hsv//eng |
| 653 | a RNA folding het-erogeneity | |
| 653 | a Pre‐miRNA | |
| 653 | a RNA‐ligand interaction | |
| 653 | a RNA dynamics | |
| 653 | a Optical tweezers | |
| 653 | a Pre‐miRNA‐377 | |
| 653 | a MiRNA | |
| 653 | a Single‐molecule force spectroscopy | |
| 653 | a Heart failure | |
| 700 | 1 | a Sundar Rajan, Vinoth,d 1987u Chalmers tekniska högskola,Chalmers University of Technology4 aut0 (Swepub:cth)edal |
| 700 | 1 | a Westerlund, Fredrik,d 1978u Chalmers tekniska högskola,Chalmers University of Technology4 aut0 (Swepub:cth)fredrikw |
| 700 | 1 | a Wilhelmsson, Marcus,d 1974u Chalmers tekniska högskola,Chalmers University of Technology4 aut0 (Swepub:cth)mawi |
| 710 | 2 | a Chalmers tekniska högskola4 org |
| 773 | 0 | t International Journal of Molecular Sciencesd : MDPI AGg 22:16q 22:16x 1661-6596x 1422-0067 |
| 856 | 4 | u https://research.chalmers.se/publication/525731/file/525731_Fulltext.pdfx primaryx freey FULLTEXT |
| 856 | 4 | u https://www.mdpi.com/1422-0067/22/16/9008/pdf |
| 856 | 4 8 | u https://research.chalmers.se/publication/525731 |
| 856 | 4 8 | u https://doi.org/10.3390/ijms22169008 |
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