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Towards a human pro...
Towards a human proteome atlas : high-throughput generation of mono-specific antibodies for tissue profiling
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- Nilsson, Peter (author)
- KTH,Proteomik
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- Paavilainen, Linda (author)
- Uppsala universitet,Institutionen för genetik och patologi,Department of Genetics and Pathology, The Rudbeck Laboratory, Uppsala University, Uppsala, Sweden
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- Larsson, Karin (author)
- KTH,Skolan för bioteknologi (BIO)
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- Ödling, Jenny (author)
- KTH,Skolan för bioteknologi (BIO)
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- Sundberg, Mårten (author)
- KTH,Proteomik,Department of Biotechnology, Royal Institute of Technology (KTH), Stockholm
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- Andersson, Ann-Catrin (author)
- Uppsala universitet,Institutionen för genetik och patologi,Department of Genetics and Pathology, The Rudbeck Laboratory, Uppsala University, Uppsala, Sweden
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- Kampf, Caroline (author)
- Uppsala universitet,Institutionen för genetik och patologi,Department of Genetics and Pathology, The Rudbeck Laboratory, Uppsala University, Uppsala, Sweden
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- Persson, Anja (author)
- KTH,Proteomik
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- Al-Khalili Szigyarto, Cristina (author)
- KTH,Proteomik
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- Ottoson, Jenny (author)
- KTH,Proteomik
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- Björling, Erik (author)
- KTH,Skolan för bioteknologi (BIO)
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- Hober, Sophia (author)
- KTH,Proteomik
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- Wernérus, Henrik (author)
- KTH,Skolan för bioteknologi (BIO)
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- Wester, Kenneth (author)
- Uppsala universitet,Institutionen för genetik och patologi,Department of Genetics and Pathology, The Rudbeck Laboratory, Uppsala University, Uppsala, Sweden
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- Ponten, Fredrik (author)
- Uppsala universitet,Institutionen för genetik och patologi,Department of Genetics and Pathology, The Rudbeck Laboratory, Uppsala University, Uppsala, Sweden
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- Uhlén, Mathias (author)
- KTH,Proteomik
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(creator_code:org_t)
- 2005-10-20
- 2005
- English.
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In: Proteomics. - : Wiley. - 1615-9853 .- 1615-9861. ; 5:17, s. 4327-4337
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Abstract
Subject headings
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- A great need exists for the systematic generation of specific antibodies to explore the human proteome. Here, we show that antibodies specific to human proteins can be generated in a high-throughput manner involving stringent affinity purification using recombinant protein epitope signature tags (PrESTs) as immunogens and affinity-ligands. The specificity of the generated affinity reagents, here called mono-specific antibodies (msAb), were validated with a novel protein microarray assay. The success rate for 464 antibodies generated towards human proteins was more than 90% as judged by the protein array assay. The antibodies were used for parallel profiling of patient biopsies using tissue microarrays generated from 48 human tissues. Comparative analysis with well-characterized monoclonal antibodies showed identical or similar specificity and expression patterns. The results suggest that a comprehensive atlas containing extensive protein expression and subcellular localization data of the human proteome can be generated in an efficient manner with mono-specific antibodies.
Subject headings
- NATURVETENSKAP -- Biologi -- Biokemi och molekylärbiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Biochemistry and Molecular Biology (hsv//eng)
- TEKNIK OCH TEKNOLOGIER -- Industriell bioteknik (hsv//swe)
- ENGINEERING AND TECHNOLOGY -- Industrial Biotechnology (hsv//eng)
Keyword
- Antibody generation
- protein microarray
- proteome atlas
- tissue microarray
- tissue profiling
- Bioengineering
Publication and Content Type
- ref (subject category)
- art (subject category)
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Proteomics
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- By the author/editor
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Nilsson, Peter
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Paavilainen, Lin ...
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Larsson, Karin
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Ödling, Jenny
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Sundberg, Mårten
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Andersson, Ann-C ...
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show more...
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Kampf, Caroline
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Persson, Anja
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Al-Khalili Szigy ...
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Ottoson, Jenny
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Björling, Erik
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Hober, Sophia
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Wernérus, Henrik
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Wester, Kenneth
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Ponten, Fredrik
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Uhlén, Mathias
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- About the subject
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- NATURAL SCIENCES
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NATURAL SCIENCES
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and Biological Scien ...
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and Biochemistry and ...
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- ENGINEERING AND TECHNOLOGY
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ENGINEERING AND ...
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and Industrial Biote ...
- Articles in the publication
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Proteomics
- By the university
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Uppsala University
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Royal Institute of Technology