WFRF:(Domeika M A)
Sökning: WFRF:(Domeika M A) > Evaluation of six n...
Evaluation of six nucleic acid amplification tests used for diagnosis of Neisseria gonorrhoeae in Russia compared with an international strictly validated real-time porA pseudogene polymerase chain reaction
- Shipitsyna, E (författare)
- Zolotoverkhaya, E (författare)
- Hjelmevoll, O (författare)
- visa fler...
- Maximova, A (författare)
- Savicheva, A (författare)
- Sokolovsky, E (författare)
- Skogen, V (författare)
- Unemo, M (författare)
- visa färre...
- (creator_code:org_t)
- Wiley, 2009
- 2009
- Engelska.
- Ingår i: Journal of the European Academy of Dermatology and Venereology. - : Wiley. - 0926-9959 .- 1468-3083. ; 23:11, s. 1246-1253
- Tidskriftsartikel (refereegranskat)
Abstract
Ämnesord
Stäng
- Background In Russia, laboratory diagnosis of gonorrhoea has been mainly based on microscopy only and, in some settings, relatively rare suboptimal culturing. In recent years, Russian developed and manufactured nucleic acid amplification tests (NAAT) have been implemented for routine diagnosis of Neisseria gonorrhoeae. However, these NAATs have never been validated to any international well-recognized diagnostic NAAT. Objective This study aims to evaluate the performance characteristics of six Russian NAATs for N. gonorrhoeae diagnostics. Materials and methods In total, 496 symptomatic patients were included. Five polymerase chain reaction (PCR) assays and one real-time nucleic acid sequence based amplification (NASBA) assay, developed by three Russian companies, were evaluated on urogenital samples, i.e. cervical and first voided urine (FVU) samples from females (n = 319), urethral and FVU samples from males (n = 127), and extragenital samples, i.e. rectal and pharyngeal samples, from 50 additional female patients with suspicion of gonorrhoea. As reference method, an international strictly validated real-time porA pseudogene PCR was applied. Results The prevalence of N. gonorrhoeae was 2.7% and 16% among the patients providing urogenital and extragenital samples, respectively. The Russian NAATs and the reference method displayed high level of concordance (99.4-100%). The sensitivities, specificities, positive predictive values and negative predictive values of the Russian tests in different specimens were 66.7-100%, 100%, 100%, and 99.4-100%, respectively. Conclusions Russian N. gonorrhoeae diagnostic NAATs comprise relatively good performance characteristics. However, larger studies are crucial and, beneficially, the Russian assays should also be evaluated to other international highly sensitive and specific, and ideally Food and Drug Administration approved, NAATs such as Aptima Combo 2 (Gen-Probe).Conflicts of interest None declared.
Nyckelord
- Neisseria gonorrhoeae
- nucleic acid amplification tests
- porA pseudogene PCR
- Russia
- sensitivity and specificity
- MEDICINE
- MEDICIN
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
Hitta via bibliotek
- Journal of the European Academy of Dermatology and Venereology (Sök värdpublikationen i LIBRIS)
Till lärosätets databas
Hitta mer i SwePub
- Av författaren/redakt...
- Shipitsyna, E
- Zolotoverkhaya, ...
- Hjelmevoll, O
- Maximova, A
- Savicheva, A
- Sokolovsky, E
- visa fler...
- Skogen, V
- Domeika, M
- Unemo, M
- visa färre...
- Artiklar i publikationen
- Journal of the E ...
- Av lärosätet
- Uppsala universitet
Sök utanför SwePub
- Sök vidare i:
- Google Book Search
- Google Scholar
Kungliga biblioteket hanterar dina personuppgifter i enlighet med EU:s dataskyddsförordning (2018), GDPR. Läs mer om hur det funkar här.
Så här hanterar KB dina uppgifter vid användning av denna tjänst.
- Copyright © LIBRIS - Nationella bibliotekssystem
- LIBRIS.kb.se
