Effects of K+-induced depolarization and purinergic receptor activation on elemental content in insulin-producing RINm5F-cells

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Fältnamn	Indikatorer	Metadata
000		03153naa a2200337 4500
001		oai:DiVA.org:uu-35342
003		SwePub
008		081017s1995 \| \|\|\|\|\|\|\|\|\|\|\|000 \|\|eng\|
009		oai:prod.swepub.kib.ki.se:1958187
024	7	a https://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-353422 URI
024	7	a https://doi.org/10.1006/cbir.1995.10042 DOI
024	7	a http://kipublications.ki.se/Default.aspx?queryparsed=id:19581872 URI
040		a (SwePub)uud (SwePub)ki
041		a engb eng
042		9 SwePub
072	7	a ref2 swepub-contenttype
072	7	a art2 swepub-publicationtype
100	1	a Pålsgård, Evau Karolinska Institutet,Uppsala universitet,Enheten för biomedicinsk strålningsvetenskap4 aut0 (Swepub:uu)epa29899
245	1 0	a Effects of K+-induced depolarization and purinergic receptor activation on elemental content in insulin-producing RINm5F-cells
264	1	b Wiley,c 1995
338		a print2 rdacarrier
520		a X-ray microanalysis was used to detect elemental changes in the insulin-producing tumor cell-lineRINm5F. To improve discrimination between mobile ions and ions bound to macromolecules a new approach was employed, consisting of multivariate statistical analysis of correlations between the concentrations of Na, Mg, P, S, CI, K, and Ca. RINm5F cells, cultured an Formvar-coated titanium grids, were stimulated with high K+ or ATP, that are both known to stimulate insulin release. The buffers used contained Ca2+ or one of the Ca2+-analogues Sr2+ and Ba2+, to represent Ca2+ uptake inresponse to stimulation. After stimulation the cells were shock-frozen and freeze-dried overnight. Incubation for 10-20 seconds in a Ca2+-containing buffer did not significantly affect elementalcomposition, whereas cellular Mg, P and K decreased in a Sr2+-containing buffer. Depolarization with high K+ concentration caused an increase in the cellular Na content, both in Ca2+- and Sr2+-containing buffers, but not in the buffer where Ca2+ had been replaced by Ba2+. X-ray microanalysis is useful for detection of elemental changes subsequent to stimulation of cultured cells. Moreover, multivariate statistical analysis strengthens the idea that stimulation of RINm5F cells causes redistribution of ions possibly due to changes in the state of binding of some elements to cellular proteins.
700	1	a Lindh, Ulfu Karolinska Institutet,Uppsala universitet,Enheten för biomedicinsk strålningsvetenskap4 aut
700	1	a Juntti-Berggren, Lisau Uppsala universitet,Enheten för biomedicinsk strålningsvetenskap4 aut
700	1	a Berggren, Per-Olof4 aut
700	1	a Roomans, Godfried Mu Uppsala universitet,Enheten för biomedicinsk strålningsvetenskap4 aut
710	2	a Uppsala universitetb Enheten för biomedicinsk strålningsvetenskap4 org
773	0	t Cell Biology Internationald : Wileyg 19:1, s. 25-34q 19:1<25-34x 1065-6995x 1095-8355
856	4 8	u https://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-35342
856	4 8	u https://doi.org/10.1006/cbir.1995.1004
856	4 8	u http://kipublications.ki.se/Default.aspx?queryparsed=id:1958187

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Av författaren/redakt...: Pålsgård, Eva; Lindh, Ulf; Juntti-Berggren, ...; Berggren, Per-Ol ...; Roomans, Godfrie ...

Artiklar i publikationen: Cell Biology Int ...

Av lärosätet: Uppsala universitet; Karolinska Institutet

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