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Fluorescence Lifeti...
Fluorescence Lifetime Imaging as an in Situ and Label-Free Readout for the Chemical Composition of Lignin
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- Escamez, Sacha, 1987- (author)
- Swedish University of Agricultural Sciences,Sveriges lantbruksuniversitet,Umeå universitet,Institutionen för fysiologisk botanik,Umeå Plant Science Centre (UPSC),Umeå Plant Science Centre (UPSC), Department of Forest Genetics and Plant Physiology, Swedish University of Agricultural Sciences, Umeå, Sweden,SLU Swedish University of Agricultural Sciences, Sweden; Umeå University, Sweden,Institutionen för skoglig genetik och växtfysiologi,Department of Forest Genetics and Plant Physiology,Umeå University
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- Terryn, C. (author)
- PICT Platform, Université de Reims Champagne Ardenne, Reims, France
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- Gandla, Madhavi Latha (author)
- Umeå universitet,Kemiska institutionen,Umeå University, Sweden
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- Yassin, Zakiya (author)
- RISE,Bygg och fastighet,RISE AB, Stockholm, Sweden
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- Scheepers, Gerhard (author)
- RISE,Bygg och fastighet,RISE AB, Stockholm, Sweden
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- Näsholm, Torgny (author)
- Swedish University of Agricultural Sciences,Sveriges lantbruksuniversitet,Institutionen för skoglig genetik och växtfysiologi,Institutionen för skogens ekologi och skötsel,Department of Forest Genetics and Plant Physiology,Department of Forest Ecology and Management
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- Sundman, Ola (author)
- Umeå universitet,Kemiska institutionen,Umeå University, Sweden
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- Jönsson, Leif J. (author)
- Umeå universitet,Kemiska institutionen,Umeå University, Sweden
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- Lundberg-Felten, Judith (author)
- Swedish University of Agricultural Sciences,Sveriges lantbruksuniversitet,Institutionen för skoglig genetik och växtfysiologi,Department of Forest Genetics and Plant Physiology
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- Tuominen, H. (author)
- Swedish University of Agricultural Sciences,Sveriges lantbruksuniversitet,Institutionen för skoglig genetik och växtfysiologi,Department of Forest Genetics and Plant Physiology
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- Niittylä, Totte (author)
- Swedish University of Agricultural Sciences,Sveriges lantbruksuniversitet,Institutionen för skoglig genetik och växtfysiologi,Department of Forest Genetics and Plant Physiology
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- Paës, G. (author)
- Université de Reims Champagne Ardenne, Reims, France
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(creator_code:org_t)
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- 2021-12-11
- 2021
- English.
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In: ACS Sustainable Chemistry and Engineering. - : American Chemical Society. - 2168-0485. ; 9:51
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https://doi.org/10.1...
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https://umu.diva-por... (primary) (Raw object)
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https://urn.kb.se/re...
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https://doi.org/10.1...
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https://urn.kb.se/re...
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https://res.slu.se/i...
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Abstract
Subject headings
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- Naturally fluorescent polymeric molecules such as collagen, resilin, cutin, suberin, or lignin can serve as renewable sources of bioproducts. Theoretical physics predicts that the fluorescence lifetime of these polymers is related to their chemical composition. We verified this prediction for lignin, a major structural element in plant cell walls that form woody biomass. Lignin is composed of different phenylpropanoid units, and its composition affects its properties, biological functions, and the utilization of wood biomass. We carried out fluorescence lifetime imaging microscopy (FLIM) measurements of wood cell wall lignin in a population of 90 hybrid aspen trees genetically engineered to display differences in cell wall chemistry and structure. We also measured the wood cell wall composition by classical analytical methods in these trees. Using statistical modeling and machine learning algorithms, we identified parameters of fluorescence lifetime that predict the content of S-type and G-type lignin units, the two main types of units in the lignin of angiosperm (flowering) plants. In a first step toward tailoring lignin biosynthesis toward improvement of woody biomass feedstocks, we show how FLIM can reveal the dynamics of lignin biosynthesis in two different biological contexts, including in vivo while lignin is being synthesized in the walls of living cells. © 2021 The Authors.
Subject headings
- LANTBRUKSVETENSKAPER -- Lantbruksvetenskap, skogsbruk och fiske -- Trävetenskap (hsv//swe)
- AGRICULTURAL SCIENCES -- Agriculture, Forestry and Fisheries -- Wood Science (hsv//eng)
- LANTBRUKSVETENSKAPER -- Bioteknologi med applikationer på växter och djur -- Växtbioteknologi (hsv//swe)
- AGRICULTURAL SCIENCES -- Agricultural Biotechnology -- Plant Biotechnology (hsv//eng)
Keyword
- chemotyping in situ
- FLIM
- lignin
- machine learning
- statistical modeling
- wood
- Biochemistry
- Biomass
- Biosynthesis
- Cells
- Cytology
- Fluorescence imaging
- Forestry
- Learning algorithms
- Statistical methods
- Chemical compositions
- Fluorescence lifetime imaging
- Fluorescence lifetime imaging microscopy
- Fluorescence lifetimes
- Label free
- Lignin biosynthesis
- Polymeric molecules
- Statistic modeling
- Wood cell walls
Publication and Content Type
- ref (subject category)
- art (subject category)
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- By the author/editor
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Escamez, Sacha, ...
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Terryn, C.
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Gandla, Madhavi ...
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Yassin, Zakiya
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Scheepers, Gerha ...
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Näsholm, Torgny
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show more...
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Sundman, Ola
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Jönsson, Leif J.
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Lundberg-Felten, ...
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Tuominen, H.
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Niittylä, Totte
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Paës, G.
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- About the subject
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- AGRICULTURAL SCIENCES
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AGRICULTURAL SCI ...
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and Agriculture Fore ...
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and Wood Science
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- AGRICULTURAL SCIENCES
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AGRICULTURAL SCI ...
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and Agricultural Bio ...
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and Plant Biotechnol ...
- Articles in the publication
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ACS Sustainable ...
- By the university
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RISE
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Umeå University
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Swedish University of Agricultural Sciences