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Characterization of...
Characterization of rna sensing pathways in hepatoma cell lines and primary human hepatocytes
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- Nicolay, Wiebke (author)
- TWINCORE—Centre for Experimental and Clinical Infection Research, Institute for Experimental Virology, Hannover, Germany
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- Moeller, Rebecca (author)
- TWINCORE—Centre for Experimental and Clinical Infection Research, Institute for Experimental Virology, Hannover, Germany; Center for Emerging Infections and Zoonoses (RIZ), Institute of Biochemistry & Research, University of Veterinary Medicine Hannover, Hannover, Germany
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- Kahl, Sina (author)
- TWINCORE—Centre for Experimental and Clinical Infection Research, Institute for Experimental Virology, Hannover, Germany
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- Vondran, Florian W. R. (author)
- Department of General, Visceral and Transplant Surgery, Hannover Medical School, Hannover, Germany; German Centre for Infection Research (DZIF), Braunschweig, Germany
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- Pietschmann, Thomas (author)
- TWINCORE—Centre for Experimental and Clinical Infection Research, Institute for Experimental Virology, Hannover, Germany
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- Kunz, Stefan (author)
- Institute of Microbiology, Lausanne University Hospital, Lausanne, Switzerland
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- Gerold, Gisa, 1979- (author)
- Umeå universitet,Wallenberg centrum för molekylär medicin vid Umeå universitet (WCMM),Avdelningen för virologi,TWINCORE—Centre for Experimental and Clinical Infection Research, Institute for Experimental Virology, Hannover, Germany; Center for Emerging Infections and Zoonoses (RIZ), Institute of Biochemistry & Research, University of Veterinary Medicine Hannover, Hannover, Germany
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(creator_code:org_t)
- 2021-11-04
- 2021
- English.
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In: Cells. - : MDPI. - 2073-4409. ; 10:11
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Abstract
Subject headings
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- The liver is targeted by several human pathogenic RNA viruses for viral replication and dissemination; despite this, the extent of innate immune sensing of RNA viruses by human hepatocytes is insufficiently understood to date. In particular, for highly human tropic viruses such as hepatitis C virus, cell culture models are needed to study immune sensing. However, several human hepatoma cell lines have impaired RNA sensing pathways and fail to mimic innate immune responses in the human liver. Here we compare the RNA sensing properties of six human hepatoma cell lines, namely Huh-6, Huh-7, HepG2, HepG2-HFL, Hep3B, and HepaRG, with primary human hepatocytes. We show that primary liver cells sense RNA through retinoic acid-inducible gene I (RIG-I) like receptor (RLR) and Toll-like receptor 3 (TLR3) pathways. Of the tested cell lines, Hep3B cells most closely mimicked the RLR and TLR3 mediated sensing in primary hepatocytes. This was shown by the expression of RLRs and TLR3 as well as the expression and release of bioactive interferon in primary hepatocytes and Hep3B cells. Our work shows that Hep3B cells partially mimic RNA sensing in primary hepatocytes and thus can serve as in vitro model to study innate immunity to RNA viruses in hepatocytes.
Subject headings
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Cell- och molekylärbiologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Cell and Molecular Biology (hsv//eng)
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Mikrobiologi inom det medicinska området (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Microbiology in the medical area (hsv//eng)
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Immunologi inom det medicinska området (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Immunology in the medical area (hsv//eng)
Keyword
- Arenavirus
- Coronavirus
- Hepatoma cells
- Innate immunity
- Interferon
- Liver
- Primary hepatocytes
- RIG-I
- RNA virus
- TLR3
Publication and Content Type
- ref (subject category)
- art (subject category)
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