Identification of AHNAK as a novel autoantigen in systemic lupus erythematosus.

• To identify candidate autoantigens associated with arthritis, a rat chondrocyte cDNA library was immunoscreened with serum from a patient with rheumatoid arthritis. One isolated cDNA encoded part of AHNAK, a 700-kDa phosphoprotein with DNA binding properties, that appears to be involved in several signal transduction pathways. Immunoreactivity against an in vitro translated human AHNAK fragment was detected in 4.6% (5/109) of patients with rheumatoid arthritis, 29.5% (18/61) of patients with systemic lupus erythematosus (SLE), and 1.2% (2/172) of blood donors. Anti-AHNAK antibodies reacted with a recombinant human AHNAK fragment and with native AHNAK from C32 cell lysates. In vitro translated AHNAK fragment could be cleaved by granzyme B and caspase-3. Anti-AHNAK positive SLE patients had a higher frequency of homogeneous antinuclear antibody staining patterns and a lower frequency of recent mucosal ulcerations. This is the first report that AHNAK can be targeted by the immune system in autoimmune disease.

Ämnesord

MEDICIN OCH HÄLSOVETENSKAP  -- Medicinska och farmaceutiska grundvetenskaper -- Immunologi inom det medicinska området (hsv//swe)

MEDICAL AND HEALTH SCIENCES  -- Basic Medicine -- Immunology in the medical area (hsv//eng)

Nyckelord

Amino Acid Sequence

Animals

Arthritis

Rheumatoid

immunology

Autoantibodies

blood

immunology

Autoantigens

genetics

immunology

metabolism

Base Sequence

Caspase 3

Caspases

metabolism

Cells

Cultured

Chondrocytes

metabolism

Escherichia coli

genetics

Female

Granzymes

Humans

Immunoblotting

Lupus Erythematosus

Systemic

diagnosis

immunology

Male

Membrane Proteins

genetics

immunology

metabolism

Middle Aged

Molecular Sequence Data

Neoplasm Proteins

genetics

immunology

metabolism

Precipitin Tests

Protein Biosynthesis

Rats

Rats

Sprague-Dawley

Recombinant Fusion Proteins

metabolism

Serine Endopeptidases

metabolism

Tumor Cells

Cultured

Publikations- och innehållstyp

ref (ämneskategori)

art (ämneskategori)