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Early Immune Respon...
Early Immune Responses to Borrelia garinii and Borrelia afzeliiin Lyme Borreliosis : Local Complement Activation in Erythema Migrans and in vitro Studies ofComplement Activation, Phagocytosis and Cytokine Profile
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- Sandholm, Kerstin (författare)
- School of Natural Sciences, Linneaus University, Kalmar
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- Henningsson, Anna J, 1972- (författare)
- Linköpings universitet,Infektionsmedicin,Hälsouniversitetet
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- Säve, Susanne (författare)
- School of Natural Sciences, Linneaus University, Kalmar
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- Nordberg, Marika (författare)
- Linköpings universitet,Infektionsmedicin,Hälsouniversitetet
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- Garpmo, Ulf (författare)
- Department of Clinical Microbiology, Kalmar County Hospital, Sweden
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- Jansson, Christian (författare)
- The Åland Borrelia Group, the Åland Islands, Finland
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- Carlsson, Sten-Anders (författare)
- The Åland Borrelia Group, the Åland Islands, Finland
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- Nyman, Dag (författare)
- The Åland Borrelia Group, the Åland Islands, Finland
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- Bergström, Sven (författare)
- Department of Molecular Biology, Umeå University, Sweden
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- Forsberg, Pia (författare)
- Linköpings universitet,Infektionsmedicin,Hälsouniversitetet
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- Ernerudh, Jan (författare)
- Linköpings universitet,Klinisk immunologi,Hälsouniversitetet
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- Nilsson Ekdahl, Kristina (författare)
- School of Natural Sciences, Linneaus University, Kalmar
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(creator_code:org_t)
- Engelska.
- Relaterad länk:
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https://urn.kb.se/re...
Abstract
Ämnesord
Stäng
- An optimal eradication of spirochetes in Lyme borreliosis depends on the early immune response, including the potent actions of the complement system. We here assessed possible differences between two Borrelia burgdorferi genospecies in their ability to activate complement, and the consequences of complement activation in terms of phagocytosis and induction of cytokines.Early local complement activation was assessed immunohistochemically in skin biopsies from patients with erythema migrans (EM) caused by B. afzelii or B. garinii. Complement activation, phagocytosis and early cytokine and chemokine release were studied in vitro by incubating clinical isolates of B. afzelii (K78 from a human skin biopsy) and B. garinii (LU59 from human cerebrospinal fluid) in human whole blood.B. afzelii and B. garinii were detected in skin biopsies from EM and deposition of C3-fragments and IgG was found adjacent to the spirochetes. In vitro, B. garinii LU59 induced higher complement activation (measured as the generation of C3a and C5b-9), while B. afzelii K78 recruited more factor H. Phagocytosis by granulocytes and monocytes was demonstrated to be largely dependent on complement activation since phagocytosis was substantially reduced by addition of the C3 inhibitor compstatin or a C5a receptor antagonist. The early cytokine and chemokine release in human blood in response to live spirochetes revealed a rapid and pronounced pro-inflammatory response, mainly associated with the Th1- and Th17-types.We conclude that complement is activated locally in the skin in EM, and that B. garinii LU59 activates complement more than B. afzelii K78. Complement activation is pivotal for efficient phagocytosis of Borrelia spirochetes, especially in early infection before specific antibodies are produced. Both B. garinii LU59 and B. afzelii K78 induce proinflammatory cytokines rapidly, but no clear differences were seen between the two genospecies in this respect.
Ämnesord
- MEDICIN OCH HÄLSOVETENSKAP -- Klinisk medicin -- Infektionsmedicin (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Clinical Medicine -- Infectious Medicine (hsv//eng)
Nyckelord
- Lyme borreliosis
- erythema migrans
- inflammation
- complement
- cytokine
- chemokine
- phagocytosis
Publikations- och innehållstyp
- vet (ämneskategori)
- ovr (ämneskategori)
- Av författaren/redakt...
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Sandholm, Kersti ...
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Henningsson, Ann ...
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Säve, Susanne
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Nordberg, Marika
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Garpmo, Ulf
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Jansson, Christi ...
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visa fler...
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Carlsson, Sten-A ...
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Nyman, Dag
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Bergström, Sven
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Forsberg, Pia
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Ernerudh, Jan
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Nilsson Ekdahl, ...
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visa färre...
- Om ämnet
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- MEDICIN OCH HÄLSOVETENSKAP
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MEDICIN OCH HÄLS ...
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och Klinisk medicin
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och Infektionsmedici ...
- Av lärosätet
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Linköpings universitet