WFRF:(Salmela E)
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Clonal culturing of human embryonic stem cells on laminin-521/E-cadherin matrix in defined and xeno-free environment
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- Rodin, S (author)
- Karolinska Institutet
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- Antonsson, L (author)
- Karolinska Institutet
- Niaudet, C (author)
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- Simonson, OE (author)
- Salmela, E (author)
- Hansson, EM (author)
- Xiao, ZJ (author)
- Sheikhi, M (author)
- Baker, D (author)
- Sun, Y (author)
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- (creator_code:org_t)
- 2014-01-27
- 2014
- English.
- In: Nature communications. - : Springer Science and Business Media LLC. - 2041-1723. ; 5, s. 3195-
- Journal article (peer-reviewed)
Abstract
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- Lack of robust methods for establishment and expansion of pluripotent human embryonic stem (hES) cells still hampers development of cell therapy. Laminins (LN) are a family of highly cell-type specific basement membrane proteins important for cell adhesion, differentiation, migration and phenotype stability. Here we produce and isolate a human recombinant LN-521 isoform and develop a cell culture matrix containing LN-521 and E-cadherin, which both localize to stem cell niches in vivo. This matrix allows clonal derivation, clonal survival and long-term self-renewal of hES cells under completely chemically defined and xeno-free conditions without ROCK inhibitors. Neither LN-521 nor E-cadherin alone enable clonal survival of hES cells. The LN-521/E-cadherin matrix allows hES cell line derivation from blastocyst inner cell mass and single blastomere cells without a need to destroy the embryo. This method can facilitate the generation of hES cell lines for development of different cell types for regenerative medicine purposes.
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- Rodin, S
- Antonsson, L
- Niaudet, C
- Simonson, OE
- Salmela, E
- Hansson, EM
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- Domogatskaya, A
- Xiao, ZJ
- Damdimopoulou, P
- Sheikhi, M
- Inzunza, J
- Nilsson, AS
- Baker, D
- Kuiper, R
- Sun, Y
- Blennow, E
- Nordenskjold, M
- Grinnemo, KH
- Kere, J
- Betsholtz, C
- Hovatta, O
- Tryggvason, K
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