Oral bacteria in biofilms exhibit slow reactivation from nutrient deprivation

↓ Direkt till sidans innehåll
↓ Direkt till sidans sekundära innehåll (sidomenyn)

Sökning: WFRF:(Svensäter Gunnel) > Oral bacteria in bi...

Chávez de Paz, Luis Eduardo (författare)

Oral bacteria in biofilms exhibit slow reactivation from nutrient deprivation

Artikel/kapitelEngelska2008

Förlag, utgivningsår, omfång ...

Microbiology Society,2008
printrdacarrier

Nummerbeteckningar

LIBRIS-ID:oai:DiVA.org:mau-6768
https://urn.kb.se/resolve?urn=urn:nbn:se:mau:diva-6768URI
https://doi.org/10.1099/mic.0.2008/016576-0DOI

Kompletterande språkuppgifter

Språk:engelska
Sammanfattning på:engelska

Ingår i deldatabas

SwePubSwePub

Klassifikation

Ämneskategori:ref swepub-contenttype
Ämneskategori:art swepub-publicationtype

Anmärkningar

The ability of oral bacteria to enter a non-growing state is believed to be an important mechanism for survival in the starved micro-environments of the oral cavity. In this study, we examined the reactivation of nutrient-deprived cells of two oral bacteria in biofilms, Streptococcus anginosus and Lactobacillus salivarius. Non-growing cells were generated by incubation in 10 mM potassium phosphate buffer for 24 h and the results were compared to those of planktonic cultures. When both types of cells were shifted from a rich, peptone-yeast extract-glucose (PYG) medium to buffer for 24 h, dehydrogenase and esterase activity measured by the fluorescent dyes 5-cyano-2,3-ditolyl-tetrazolium chloride (CTC) and fluorescein diacetate (FDA), respectively, was absent in both species. However, the membranes of the vast majority of nutrient-deprived cells remained intact as assessed by LIVE/DEAD staining. Metabolic reactivation of the nutrient-deprived biofilm cells was not observed for at least 48 h following addition of fresh PYG medium, whereas the non-growing planktonic cultures of the same two strains were in rapid growth in less than 2 h. At 72 h, the S. anginosus biofilm cells had recovered 78 % of the dehydrogenase activity and 61 % of the esterase activity and the biomass mm(-2) had increased by 30-35 %. With L. salivarius at 72 h, the biofilms had recovered 56 % and 75 % of dehydrogenase and esterase activity, respectively. Reactivation of both species in biofilms was enhanced by removal of glucose from PYG, and S. anginosus cells were particularly responsive to yeast extract (YE) medium. The data suggest that the low reactivity of non-growing biofilm cells to the introduction of fresh nutrients may be a survival strategy employed by micro-organisms in the oral cavity.

Ämnesord och genrebeteckningar

MEDICIN OCH HÄLSOVETENSKAP Klinisk medicin Odontologi hsv//swe
MEDICAL AND HEALTH SCIENCES Clinical Medicine Dentistry hsv//eng

Biuppslag (personer, institutioner, konferenser, titlar ...)

Hamilton, Ian R (författare)
Svensäter, GunnelMalmö högskola,Odontologiska fakulteten (OD)(Swepub:mau)odgusv (författare)
Malmö högskolaOdontologiska fakulteten (OD) (creator_code:org_t)

Sammanhörande titlar

Ingår i:Microbiology: Microbiology Society154, s. 1927-19381350-08721465-2080

Internetlänk

Hitta via bibliotek

Microbiology (Sök värdpublikationen i LIBRIS)

Till lärosätets databas

Hitta mer i SwePub

Av författaren/redakt...: Chávez de Paz, L ...; Hamilton, Ian R; Svensäter, Gunne ...

Om ämnet

MEDICIN OCH HÄLSOVETENSKAP: MEDICIN OCH HÄLS ...; och Klinisk medicin; och Odontologi

Artiklar i publikationen: Microbiology

Av lärosätet: Malmö universitet

Sök utanför SwePub

Sök vidare i:: Google; Google Book Search; Google Scholar

Kungliga biblioteket hanterar dina personuppgifter i enlighet med EU:s dataskyddsförordning (2018), GDPR. Läs mer om hur det funkar här.
Så här hanterar KB dina uppgifter vid användning av denna tjänst.

LIBRIS.kb.se