Multifeed simultaneous saccharification and fermentation enables high gravity submerged fermentation of lignocellulose.

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Sökning: WFRF:(Tomas Pejo Elia 1980) > Multifeed simultane...

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Fältnamn	Indikatorer	Metadata
000		04526naa a2200469 4500
001		oai:research.chalmers.se:6b8990dd-0d92-4992-8825-b298b9cfdc18
003		SwePub
008		171007s2015 \| \|\|\|\|\|\|\|\|\|\|\|000 \|\|eng\|
024	7	a https://research.chalmers.se/publication/2276142 URI
040		a (SwePub)cth
041		a engb eng
042		9 SwePub
072	7	a kon2 swepub-publicationtype
072	7	a vet2 swepub-contenttype
100	1	a Franzén, Carl Johan,d 1966u Chalmers tekniska högskola,Chalmers University of Technology4 aut0 (Swepub:cth)franzen
245	1 0	a Multifeed simultaneous saccharification and fermentation enables high gravity submerged fermentation of lignocellulose.
264	1	c 2015
520		a Today, second generation bioethanol production is becoming established in production plants across the world. In addition to its intrinsic value, the process can be viewed as a model process for biotechnological conversion of recalcitrant lignocellulosic raw materials to a range of chemicals and other products. So called High Gravity operation, i.e. fermentation at high solids loadings, represents continued development of the process towards higher product concentrations and productivities, and improved energy and water economy. We have employed a systematic, model-driven approach to the design of feeding schemes of solid substrate, active yeast adapted to the actual substrate, and enzymes to fed-batch simultaneous saccharification and co-fermentation (Multifeed SSCF) of steam-pretreated lignocellulosic materials in stirred tank reactors. With this approach, mixing problems were avoided even at water insoluble solids contents of 22%, leading to ethanol concentrations of 56 g/L within 72 hours of SSCF on wheat straw. Similar fermentation performance was verified in 10 m3 demonstration scale using wheat straw, and in lab scale on birch and spruce, using several yeast strains. The yeast was propagated in the liquid fraction obtained by press filtration of the pretreated slurry. Yet, even with such preadaptation and repeated addition of fresh cells, the viability in the SSCF dropped due to interactions between lignocellulose-derived inhibitors, the produced ethanol and the temperature. Decreasing the temperature from 35 to 30°C when the ethanol concentration reached 40-50 g/L resulted in rapid initial hydrolysis, maintained fermentation capacity, lower residual glucose and xylose and ethanol concentrations above 60 g/L.
650	7	a TEKNIK OCH TEKNOLOGIERx Industriell bioteknik0 (SwePub)2092 hsv//swe
650	7	a ENGINEERING AND TECHNOLOGYx Industrial Biotechnology0 (SwePub)2092 hsv//eng
650	7	a TEKNIK OCH TEKNOLOGIERx Industriell bioteknikx Bioprocessteknik0 (SwePub)209012 hsv//swe
650	7	a ENGINEERING AND TECHNOLOGYx Industrial Biotechnologyx Bioprocess Technology0 (SwePub)209012 hsv//eng
650	7	a TEKNIK OCH TEKNOLOGIERx Industriell bioteknikx Bioenergi0 (SwePub)209042 hsv//swe
650	7	a ENGINEERING AND TECHNOLOGYx Industrial Biotechnologyx Bioenergy0 (SwePub)209042 hsv//eng
650	7	a TEKNIK OCH TEKNOLOGIERx Industriell bioteknikx Annan industriell bioteknik0 (SwePub)209992 hsv//swe
650	7	a ENGINEERING AND TECHNOLOGYx Industrial Biotechnologyx Other Industrial Biotechnology0 (SwePub)209992 hsv//eng
653		a biorefinery
653		a kinetic modeling
653		a flocculation
653		a bioethanol
653		a scale-up
653		a Lignocellulose
653		a fed-batch high gravity
700	1	a Wang, Ruifei,d 1985u Chalmers tekniska högskola,Chalmers University of Technology4 aut0 (Swepub:cth)ruifei
700	1	a Westman, Johan,d 1983u Chalmers tekniska högskola,Chalmers University of Technology4 aut0 (Swepub:cth)johanwe
700	1	a Xiros, Charilaos,d 1973u Chalmers tekniska högskola,Chalmers University of Technology4 aut0 (Swepub:cth)xiros
700	1	a Koppram, Rakesh,d 1986u Chalmers tekniska högskola,Chalmers University of Technology4 aut0 (Swepub:cth)koppram
700	1	a Tomas-Pejo, Elia,d 1980u Chalmers tekniska högskola,Chalmers University of Technology4 aut0 (Swepub:cth)elia
700	1	a Olsson, Lisbeth,d 1963u Chalmers tekniska högskola,Chalmers University of Technology4 aut0 (Swepub:cth)lisbetho
710	2	a Chalmers tekniska högskola4 org
773	0	t Recent Advances in Fermentation Technology (RAFT 11), Clearwater Beach, Florida, USA, November 8-11, 2015. Oral presentation.
856	4 8	u https://research.chalmers.se/publication/227614

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