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Organic Phosphorus ...
Organic Phosphorus Compounds in Aquatic Sediments : Towards Molecular Identification with Mass Spectrometry
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- De Brabandere, Heidi, 1980- (författare)
- Uppsala universitet,Institutionen för fysikalisk och analytisk kemi
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- Waldebäck, Monica, Ph.D. (preses)
- Uppsala universitet,Institutionen för fysikalisk och analytisk kemi
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- Rydin, Emil, Ph.D. (preses)
- Uppsala universitet,Institutionen för ekologi och evolution
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- Sjöberg, Per J. R., Ph.D. (preses)
- Uppsala universitet,Institutionen för fysikalisk och analytisk kemi
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- Danielsson, Rolf, Associate Professor (preses)
- Uppsala universitet,Institutionen för fysikalisk och analytisk kemi
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- Cooper, William T., Professor (opponent)
- Analytical and Environmental Chemistry, Dept. of Chemistry and Biochemistry, Florida State University
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(creator_code:org_t)
- ISBN 9789155473068
- Uppsala : Universitetsbiblioteket, 2008
- Engelska 58 s.
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Serie: Digital Comprehensive Summaries of Uppsala Dissertations from the Faculty of Science and Technology, 1651-6214 ; 560
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https://uu.diva-port... (primary) (Raw object)
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Abstract
Ämnesord
Stäng
- Phosphorus (P) regulates trophic status in most aquatic systems. However, only bioavailable P contributes to primary production. In most lakes and shallow seas, mineralisation of sediment P into its bioavailable form and its release to the water column is important for maintaining primary production. Sediment organic P forms a substantial proportion of this P to be mineralised and can originate from different sources on land (farmland, forests, etc.) or from primary production in the lake. These organic P forms can thus be expected to have differing composition, degradability and recyclable P content. Knowledge of the chemical structure of sediment organic P compounds is scarce, mainly due to lack of appropriate analytical techniques. The commonly used 31P-nuclear magnetic resonance (31P-NMR) technique, only identifies P binding groups, so a mass spectrometric (MS) analysis method was developed that allows individual sediment organic P compounds to be identified. EDTA as pre-extractant resulted in the highest P yield in subsequent NaOH extraction. Extracted organic P compound groups were identified using 31P-NMR. For identification of specific P compounds with MS, a sample preparation method prior to electrospray tandem mass spectrometry (ESI-MS/MS) analysis was developed. Liquid chromatography (LC) with porous graphitic carbon prior to ESI-MS/MS enhanced sensitivity and selectivity, enabling several of the ions detected to be identified as nucleotides. 31P-NMR analysis showed P monoesters to be the most stabile P compounds throughout a lake sediment profile. The developed LC-ESI-MS/MS analysis method revealed that some monoester-P (nucleotides) were labile, while other P compounds increased in concentration with Baltic Sea sediment depth and were therefore considered stabile. Differences in patterns of P compounds detected were also shown depending on catchment characteristics in relation to Baltic Sea sediment age. For cost-effective management of eutrophication, knowledge of the sources of degradable organic P forms, contributing to internal loading, is needed. This thesis showed the developed LC-ESI-MS/MS analysis method to be a powerful analytical tool for this purpose.
Ämnesord
- NATURVETENSKAP -- Kemi -- Analytisk kemi (hsv//swe)
- NATURAL SCIENCES -- Chemical Sciences -- Analytical Chemistry (hsv//eng)
Nyckelord
- electrospray ionisation
- mass spectrometry
- liquid chromatography
- organic phosphorus
- aquatic sediment
- eutrophication
- sample preparation
- extraction
- Analytical chemistry
- Analytisk kemi
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