onr:"swepub:oai:DiVA.org:oru-48135"
Sökning: onr:"swepub:oai:DiVA.org:oru-48135" > The value of zebraf...
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| Fältnamn | Indikatorer | Metadata |
|---|---|---|
| 000 | 05926naa a2200421 4500 | |
| 001 | oai:DiVA.org:oru-48135 | |
| 003 | SwePub | |
| 008 | 160209s2015 | |||||||||||000 ||eng| | |
| 024 | 7 | a https://urn.kb.se/resolve?urn=urn:nbn:se:oru:diva-481352 URI |
| 024 | 7 | a https://doi.org/10.1186/s12302-015-0040-y2 DOI |
| 040 | a (SwePub)oru | |
| 041 | a engb eng | |
| 042 | 9 SwePub | |
| 072 | 7 | a ref2 swepub-contenttype |
| 072 | 7 | a for2 swepub-publicationtype |
| 100 | 1 | a Di Paolo, Carolinau Department of Ecosystem Analysis, Institute for Environmental Research, ABBt - Aachen Biology and Biotechnology, RWTH Aachen University, Aachen, Germany4 aut |
| 245 | 1 0 | a The value of zebrafish as an integrative model in effect-directed analysis :b a review |
| 264 | c 2015-03-14 | |
| 264 | 1 | b Springer,c 2015 |
| 338 | a print2 rdacarrier | |
| 520 | a Bioassays play a central role in effect-directed analysis (EDA), and their selection and application have to consider rather specific aspects of this approach. Meanwhile, bioassays with zebrafish, an established model organism in different research areas, are increasingly being utilized in EDA. Aiming to contribute for the optimal application of zebrafish bioassays in EDA, this review provides a critical overview of previous EDA investigations that applied zebrafish bioassays, discusses the potential contribution of such methods for EDA and proposes strategies to improve future studies. Over the last 10 years, zebrafish bioassays have guided EDA of natural products and environmental samples. The great majority of studies performed bioassays with embryos and early larvae, which allowed small-scale and low-volume experimental setups, minimized sample use and reduced workload. Biotesting strategies applied zebrafish bioassays as either the only method guiding EDA or instead integrated into multiple bioassay approaches. Furthermore, tiered biotesting applied zebrafish methods in both screening phase as well as for further investigations. For dosing, most of the studies performed solvent exchange of extracts and fractions to dimethyl sulfoxide (DMSO) as carrier. However, high DMSO concentrations were required for the testing of complex matrix extracts, indicating that future studies might benefit from the evaluation of alternative carrier solvents or passive dosing. Surprisingly, only a few studies reported the evaluation of process blanks, indicating a need to improve and standardize methods for blank preparation and biotesting. Regarding evaluated endpoints, while acute toxicity brought limited information, the assessment of specific endpoints was of strong value for bioactivity identification. Therefore, the bioassay specificity and sensitivity to identify the investigated bioactivity are important criteria in EDA. Additionally, it might be necessary to characterize the most adequate exposure windows and assessment setups for bioactivity identification. Finally, a great advantage of zebrafish bioassays in EDA of environmental samples is the availability of mechanism- and endpoint-specific methods for the identification of important classes of contaminants. The evaluation of mechanism-specific endpoints in EDA is considered to be a promising strategy to facilitate the integration of EDA into weight-of-evidence approaches, ultimately contributing for the identification of environmental contaminants causing bioassay and ecological effects. | |
| 650 | 7 | a NATURVETENSKAPx Geovetenskap och miljövetenskapx Miljövetenskap0 (SwePub)105022 hsv//swe |
| 650 | 7 | a NATURAL SCIENCESx Earth and Related Environmental Sciencesx Environmental Sciences0 (SwePub)105022 hsv//eng |
| 653 | a Effect-directed analysis; Bioassay-guided fractionation; Zebrafish; Embryo; Larva; Bioassay; In vitro ; In vivo | |
| 653 | a Enviromental Science | |
| 653 | a Miljövetenskap | |
| 700 | 1 | a Seiler, Thomas B.u Department of Ecosystem Analysis, Institute for Environmental Research, ABBt - Aachen Biology and Biotechnology, RWTH Aachen University, Aachen, Germany4 aut |
| 700 | 1 | a Keiter, Steffen,d 1971-u Örebro universitet,Institutionen för naturvetenskap och teknik,Department of Ecosystem Analysis, Institute for Environmental Research, ABBt - Aachen Biology and Biotechnology, RWTH Aachen University, Aachen, Germany,MTM4 aut0 (Swepub:oru)sfkr |
| 700 | 1 | a Hu, Mengu Helmholtz-Zentrum für Umweltforschung (UFZ), Helmholtz Centre for Environmental Research, Leipzig, Germany4 aut |
| 700 | 1 | a Muz, Melisu Helmholtz-Zentrum für Umweltforschung (UFZ), Helmholtz Centre for Environmental Research, Leipzig, Germany4 aut |
| 700 | 1 | a Brack, Werneru Helmholtz-Zentrum für Umweltforschung (UFZ), Helmholtz Centre for Environmental Research, Leipzig, Germany4 aut |
| 700 | 1 | a Hollert, Henneru Department of Ecosystem Analysis, Institute for Environmental Research, ABBt - Aachen Biology and Biotechnology, RWTH Aachen University, Aachen, Germany; College of Resources and Environmental Science, Chongqing University Beibei, Chongqing, China; College of Environmental Science and Engineering and State Key Laboratory of Pollution Control and Resource Reuse, Tongji University, Shanghai, China; State Key Laboratory of Pollution Control and Resource Reuse, School of the Environment, Nanjing University, Nanjing, China4 aut |
| 710 | 2 | a Department of Ecosystem Analysis, Institute for Environmental Research, ABBt - Aachen Biology and Biotechnology, RWTH Aachen University, Aachen, Germanyb Institutionen för naturvetenskap och teknik4 org |
| 773 | 0 | t Environmental Sciences Europed : Springerg 27:8, s. 1-11q 27:8<1-11x 2190-4707x 2190-4715 |
| 856 | 4 | u https://doi.org/10.1186/s12302-015-0040-yy Fulltext |
| 856 | 4 | u https://enveurope.springeropen.com/track/pdf/10.1186/s12302-015-0040-y |
| 856 | 4 8 | u https://urn.kb.se/resolve?urn=urn:nbn:se:oru:diva-48135 |
| 856 | 4 8 | u https://doi.org/10.1186/s12302-015-0040-y |
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