Sökning: (L773:0095 1137) lar1:(lu) >
High-resolution gen...
High-resolution genotyping of Chlamydia trachomatis strains by multilocus sequence analysis
-
- Klint, Markus (författare)
- Uppsala universitet,Institutionen för medicinska vetenskaper
-
- Fuxelius, Hans-Henrik (författare)
- Uppsala universitet,Molekylär evolution
-
- Goldkuhl, Renée Röstlinger (författare)
- Uppsala universitet,Institutionen för neurovetenskap
-
visa fler...
-
- Skarin, Hanna (författare)
- Uppsala universitet,Institutionen för medicinska vetenskaper
-
- Rutemark, Christian (författare)
- Uppsala universitet,Institutionen för medicinsk biokemi och mikrobiologi
-
- Andersson, Siv G. E. (författare)
- Uppsala universitet,Molekylär evolution
-
- Persson, Kenneth (författare)
- Lund University,Lunds universitet,Klinisk mikrobiologi, Malmö,Forskargrupper vid Lunds universitet,Clinical Microbiology, Malmö,Lund University Research Groups
-
- Herrmann, Björn (författare)
- Uppsala universitet,Institutionen för medicinska vetenskaper
-
visa färre...
-
(creator_code:org_t)
- 2007
- 2007
- Engelska.
-
Ingår i: Journal of Clinical Microbiology. - 0095-1137 .- 1098-660X. ; 45:5, s. 1410-1414
- Relaterad länk:
-
http://www.ncbi.nlm.... (free)
-
visa fler...
-
http://dx.doi.org/10... (free)
-
https://urn.kb.se/re...
-
https://doi.org/10.1...
-
https://lup.lub.lu.s...
-
visa färre...
Abstract
Ämnesord
Stäng
- Genotyping of Chlamydia trachomatis is limited by the low sequence variation in the genome, and no adequatemethod is available for analysis of the spread of chlamydial infections in the community. We have developeda multilocus sequence typing (MLST) system based on five target regions and compared it with analysis ofompA, the single gene most extensively used for genotyping. Sequence determination of 16 reference strains,comprising all major serotypes, serotypes A to L3, showed that the number of genetic variants in the fiveseparate target regions ranged from 8 to 16. The genetic variation in 47 clinical C. trachomatis isolates ofrepresentative serotypes (14 serotype D, 12 serotype E, 11 serotype G, and 10 serotype K strains) was analyzed;and the MLST system detected 32 variants, whereas 12 variants were detected by using ompA analysis.Specimens of the predominant serotype, serotype E, were differentiated into seven genotypes by MLST but intoonly two by ompA analysis. The MLST system was applied to C. trachomatis specimens from a population ofmen who have sex with men and was able to differentiate 10 specimens of one predominant ompA genotype Gvariant into four distinct MLST variants. To conclude, our MLST system can be used to discriminate C.trachomatis strains and can be applied to high-resolution molecular epidemiology.
Ämnesord
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Mikrobiologi inom det medicinska området (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Microbiology in the medical area (hsv//eng)
Nyckelord
- MEDICINE
- MEDICIN
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
Hitta via bibliotek
Till lärosätets databas