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Fluorescence enhanc...
Fluorescence enhancement from single DNA molecules confined in SiO 2 nanochannels
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- Westerlund, Fredrik, 1978 (author)
- Gothenburg University,Göteborgs universitet,Institutionen för fysik (GU),Department of Physics (GU),Chalmers tekniska högskola,Chalmers University of Technology,University of Gothenburg
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- Persson, Fredrik, 1979 (author)
- Lund University,Lunds universitet,Gothenburg University,Göteborgs universitet,Institutionen för fysik (GU),Department of Physics (GU),University of Gothenburg,Fysiska institutionen,Institutioner vid LTH,Lunds Tekniska Högskola,Department of Physics,Departments at LTH,Faculty of Engineering, LTH,Technical University of Denmark
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- Kristensen, A. (author)
- Danmarks Tekniske Universitet,Technical University of Denmark
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- Tegenfeldt, Jonas O. (author)
- Lund University,Lunds universitet,Gothenburg University,Göteborgs universitet,Institutionen för fysik (GU),Department of Physics (GU),University of Gothenburg,NanoLund: Centre for Nanoscience,Annan verksamhet, LTH,Lunds Tekniska Högskola,Fasta tillståndets fysik,Fysiska institutionen,Institutioner vid LTH,Other operations, LTH,Faculty of Engineering, LTH,Solid State Physics,Department of Physics,Departments at LTH,Faculty of Engineering, LTH
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(creator_code:org_t)
- ISBN 9781618390622
- 2010
- 2010
- English.
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In: 14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010; Groningen; Netherlands; 3 October 2010 through 7 October 2010. - 9781618390622
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Abstract
Subject headings
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- We demonstrate that the detected emission intensity from YOYO-labeled DNA molecules confined in 180 nm deep Si/SiO2 nanofunnels changes significantly and not monotonically with the width of the funnel, an emission enhancement that is only detected for emitted light polarized parallel to the channel. We explain the enhancement effect as being due to optical phenomena in the channels. The enhancement effect may be of importance for quantitative fluorescence microscopy and for experiments with a tight photon budget.
Subject headings
- NATURVETENSKAP -- Fysik (hsv//swe)
- NATURAL SCIENCES -- Physical Sciences (hsv//eng)
- NATURVETENSKAP -- Fysik -- Atom- och molekylfysik och optik (hsv//swe)
- NATURAL SCIENCES -- Physical Sciences -- Atom and Molecular Physics and Optics (hsv//eng)
Keyword
- DNA
- Fluorescence microscopy
- Nanofluidics
- Single molecule
- Emission enhancement
- Emission intensity
- Enhancement effects
- Fluorescence enhancement
- Optical phenomena
- Quantitative fluorescence microscopy
- Single DNA molecules
- Molecules
- Emission enhancement
Publication and Content Type
- ref (subject category)
- kon (subject category)
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