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Skin barrier disruption by sodium lauryl sulfate-exposure alters the expressions of involucrin, transglutaminase 1, profilaggrin, and kallikreins during the repair phase in human skin in vivo
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- Törmä, Hans (author)
- Uppsala universitet,Dermatologi och venereologi
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- Lindberg, Magnus (author)
- Karolinska Institutet,Örebro universitet,Hälsoakademin
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- Berne, Berit (author)
- Uppsala universitet,Dermatologi och venereologi
- (creator_code:org_t)
- London : Nature publishing group, 2008
- 2008
- English.
- In: Journal of Investigative Dermatology. - London : Nature publishing group. - 0022-202X .- 1523-1747. ; 128:5, s. 1212-1219
- Journal article (peer-reviewed)
Abstract
Subject headings
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- Detergents are skin irritants affecting keratinocytes. In this study, healthy volunteers were exposed to water (vehicle) and 1% sodium lauryl sulfate (SLS) under occlusive patch tests for 24 hours. The messenger RNA (mRNA) expression of keratinocyte differentiation markers and of enzymes involved in corneodesmosome degradation was examined in skin biopsies (n=8) during the repair phase (6 hours to 7 days postexposure) using real-time reverse-transcription PCR. It was found that the expression of involucrin was increased at 6 hours, but then rapidly normalized. The expression of transglutaminase 1 exhibited a twofold increase after 24 hours in the SLS-exposed skin. Profilaggrin was decreased after 6 hours. Later (4–7 days), the expression in SLS-exposed areas was >50% above than in control areas. An increased and altered immunofluorescence pattern of involucrin, transglutaminase 1, and filaggrin was also found (n=4). At 6 hours post-SLS exposure, the mRNA expression of kallikrein-7 (KLK-7) and kallikrein-5 (KLK-5) was decreased by 50 and 75%, respectively, as compared with control and water-exposed areas. Thereafter, the expression pattern of KLK-7 and KLK-5 was normalized. Changes in protein expression of KLK-5 were also found. In conclusion, SLS-induced skin barrier defects induce altered mRNA expression of keratinocyte differentiation markers and enzymes degrading corneodesmosomes.
Subject headings
- NATURVETENSKAP -- Matematik (hsv//swe)
- NATURAL SCIENCES -- Mathematics (hsv//eng)
- MEDICIN OCH HÄLSOVETENSKAP -- Klinisk medicin -- Dermatologi och venereologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Clinical Medicine -- Dermatology and Venereal Diseases (hsv//eng)
Keyword
- Adult
- Biopsy
- Cell Differentiation/drug effects
- Dermatitis; Irritant/pathology/*physiopathology
- Female
- Humans
- Intermediate Filament Proteins/*genetics/metabolism
- Irritants/toxicity
- Kallikreins/*genetics/metabolism
- Keratinocytes/drug effects/metabolism/pathology
- Male
- Middle Aged
- Patch Tests
- Protein Precursors/*genetics/metabolism
- RNA; Messenger/metabolism
- Reverse Transcriptase Polymerase Chain Reaction
- Skin/metabolism/pathology/physiopathology
- Sodium Dodecyl Sulfate/toxicity
- Transglutaminases/*genetics/metabolism
- Wound Healing/physiology
- MATHEMATICS
- MATEMATIK
- Dermatology and venerology
- Dermatologi och venerologi
- Dermatology and Venerology
- Dermatologi och venereologi
- MEDICINE
Publication and Content Type
- ref (subject category)
- art (subject category)
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