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A strategy for the ...
A strategy for the identification of protein architectures directly from ion mobility mass spectrometry data reveals stabilizing subunit interactions in light harvesting complexes
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- Kaldmae, Margit (author)
- Karolinska Institutet
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- Sahin, Cagla (author)
- Karolinska Institutet
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- Saluri, Mihkel (author)
- Tallinn Univ, Sch Nat Sci & Hlth, Narva Mnt 25, EE-10120 Tallinn, Estonia
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- Marklund, Erik, Teknologie doktor, 1979- (author)
- Uppsala universitet,Biokemi
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- Landreh, Michael (author)
- Karolinska Institutet
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(creator_code:org_t)
- 2019-04-19
- 2019
- English.
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In: Protein Science. - : WILEY. - 0961-8368 .- 1469-896X. ; 28:6, s. 1024-1030
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Abstract
Subject headings
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- Biotechnological applications of protein complexes require detailed information about their structure and composition, which can be challenging to obtain for proteins from natural sources. Prominent examples are the ring-shaped phycoerythrin (PE) and phycocyanin (PC) complexes isolated from the light-harvesting antennae of red algae and cyanobacteria. Despite their widespread use as fluorescent probes in biotechnology and medicine, the structures and interactions of their noncrystallizable central subunits are largely unknown. Here, we employ ion mobility mass spectrometry to reveal varying stabilities of the PC and PE complexes and identify their closest architectural homologues among all protein assemblies in the Protein Data Bank (PDB). Our results suggest that the central subunits of PC and PE complexes, although absent from the crystal structures, may be crucial for their stability, and thus of unexpected importance for their biotechnological applications.
Subject headings
- NATURVETENSKAP -- Biologi -- Biokemi och molekylärbiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Biochemistry and Molecular Biology (hsv//eng)
- NATURVETENSKAP -- Biologi -- Strukturbiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Structural Biology (hsv//eng)
Keyword
- structural mass spectrometry
- protein interactions
- collision cross sections
- protein complex stability
- ion mobility
- red algae
Publication and Content Type
- ref (subject category)
- art (subject category)
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