L773:1535 9484
Search: L773:1535 9484 > In situ proximity l...
- 8 of 219
- Previous record
- Next record
- To hitlist
In situ proximity ligation detection of c-Jun/AP-1 dimers reveals increased levels of c-Jun/Fra1 complexes in aggressive breast cancer cell lines in vitro and in vivo
- Baan, Bart (author)
- Pardali, Evangelia (author)
-
- ten Dijke, Peter (author)
- Uppsala universitet,Ludwiginstitutet för cancerforskning
- show more...
- van Dam, Hans (author)
- show less...
- (creator_code:org_t)
- 2010
- 2010
- English.
- In: Molecular & Cellular Proteomics. - 1535-9476 .- 1535-9484. ; 9:9, s. 1982-1990
- Journal article (peer-reviewed)
Abstract
Subject headings
Close
- Genetic and biochemical studies have shown that selective interactions between the Jun, Fos, and activating transcription factor (ATF) components of transcription factor activating protein 1 (AP-1) exhibit specific and critical functions in the regulation of cell proliferation, differentiation, and survival. For instance, the ratio between c-Jun/c-Fos and c-Jun/ATF2 dimers in the cell can be a determining factor in the cellular response to oncogenic or apoptotic stimuli. Until recently, no methods were available to detect endogenous AP-1 complexes in cells and tissues in situ. Here, we validated the proximity ligation assay (PLA) for its ability to specifically visualize and quantify changes in endogenous c-Jun/c-Fos, c-Jun/ATF2, and c-Jun/Fra1 complexes by using, among others, partner-selective c-Jun mutants. Furthermore, we examined the levels of c-Jun/AP-1 dimers in cell lines representing different types of human breast cancer and found that aggressive basal-like breast cancer cells can be discriminated from much less invasive luminal-like cells by PLA detection of c-Jun/Fra1 rather than of c-Jun/ATF2 and c-Jun/c-Fos. Also in tumor tissue derived from highly metastatic basal-like MDA-MB231 cells, high levels of c-Jun/Fra1 complexes were detected. Together, these results demonstrate that in situ PLA is a powerful diagnostic tool to analyze and quantify the amounts of biologically critical AP-1 dimers in fixed cells and tissue material.
Keyword
- MEDICINE
- MEDICIN
Publication and Content Type
- ref (subject category)
- art (subject category)
Find in a library
- Molecular & Cellular Proteomics (Search for host publication in LIBRIS)
To the university's database
- 8 of 219
- Previous record
- Next record
- To hitlist
Find more in SwePub
- By the author/editor
- Baan, Bart
- Pardali, Evangel ...
- ten Dijke, Peter
- van Dam, Hans
- Articles in the publication
- Molecular & Cell ...
- By the university
- Uppsala University
Search outside SwePub
- Extend your search to:
- Google Book Search
- Google Scholar
Kungliga biblioteket hanterar dina personuppgifter i enlighet med EU:s dataskyddsförordning (2018), GDPR. Läs mer om hur det funkar här.
Så här hanterar KB dina uppgifter vid användning av denna tjänst.
- Copyright © LIBRIS - National Library Systems
- LIBRIS.kb.se
