Use of novel bone biopsy system to study molecular effects of growth hormone in human bone: a pilot study

Melhus, HåkanUppsala universitet,Institutionen för medicinska vetenskaper,osteo (författare)

Use of novel bone biopsy system to study molecular effects of growth hormone in human bone : a pilot study

In this study, we have examined whether a novel bone biopsy system combined with reverse transcription-polymerase chain reaction (RT-PCR) or differential display PCR (ddPCR) can be used to detect specific mRNAs induced by growth hormone (GH) in human bone. In a 58-year-old man with complete GH deficiency as a result of empty sella, bone biopsies were taken before, and 5 and 24 h after administration of 24 recombinant human GH. Insulin-like growth factor binding protein-3 (IGFBP-3) mRNA levels in this patient, measured in a semiquantitative RT-PCR assay, increased about 40% 24 h after GH administration. This increase was not seen in a healthy control who did not receive GH, suggesting that the increase was an effect of GH rather than of the biopsy itself. Several differentially expressed mRNAs were detected by ddPCR. Thus, this pilot study suggests that our novel bone biopsy system may be suitable for in vivo studies of the molecular effects of substances with essential functions in human bone.

Åström, GunnarUppsala universitet,Enheten för radiologi (författare)
Ahlström, HåkanUppsala universitet,Enheten för radiologi (författare)
Burman, PiaUppsala universitet,Institutionen för medicinska vetenskaper (författare)
Karlsson, AndersUppsala universitet,Institutionen för medicinska vetenskaper (författare)
Ljunghall, SverkerUppsala universitet,Institutionen för medicinska vetenskaper,osteo (författare)
Uppsala universitetInstitutionen för medicinska vetenskaper (creator_code:org_t)

Ingår i:IUBMB Life - A Journal of the International Union of Biochemistry and Molecular Biology: Wiley48:2, s. 175-1781521-65431521-6551

Av författaren/redakt...: Melhus, Håkan; Åström, Gunnar; Ahlström, Håkan; Burman, Pia; Karlsson, Anders; Ljunghall, Sverk ...

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