Sökning: WFRF:(Cilla G) >
Chlamydia trachomat...
Chlamydia trachomatis genotypes A and B from urogenital specimens of patients in Spain : molecular characterization
-
- Pineiro, L. (författare)
- Hosp Univ Donostia, Inst Invest Sanitaria Biodonostia, Microbiol Dept, San Sebastian, Spain
-
- Isaksson, Jenny (författare)
- Uppsala universitet,Klinisk mikrobiologi
-
- Zapico, M. (författare)
- Hosp Univ Donostia, Inst Invest Sanitaria Biodonostia, Microbiol Dept, San Sebastian, Spain
-
visa fler...
-
- Cilla, G. (författare)
- Hosp Univ Donostia, Inst Invest Sanitaria Biodonostia, Microbiol Dept, San Sebastian, Spain;Biomed Res Ctr Network Resp Dis CIBERES, San Sebastian, Spain
-
- Herrmann, Björn, 1955- (författare)
- Uppsala universitet,Klinisk mikrobiologi
-
visa färre...
-
(creator_code:org_t)
- ELSEVIER SCI LTD, 2018
- 2018
- Engelska.
-
Ingår i: Clinical Microbiology and Infection. - : ELSEVIER SCI LTD. - 1198-743X .- 1469-0691. ; 24:8, s. 910.e5-910.e8
- Relaterad länk:
-
https://doi.org/10.1...
-
visa fler...
-
https://urn.kb.se/re...
-
https://doi.org/10.1...
-
visa färre...
Abstract
Ämnesord
Stäng
- Objectives: Chlamydia trachomatis ompA genotypes A and B, primarily associated with trachoma, were unexpectedly detected in urogenital samples of patients in Spain, a trachoma-free country. In this study, we aimed to explain this finding using analysis of organotropism-related genes and a multilocus sequence typing (MLST) technique.Methods: C trachomatis genotypes A or B were detected in 8/930 (0.9%) infection episodes between 2006 and 2012. In these strains, organotropism-related genes (polymorphic membrane protein gene H, tryptophan synthase gene A, CTA0934, and cytotoxin) were studied. Further, the strains were analysed by MLST, using a polymerase chain reaction that amplifies five highly variable genomic loci (hctB, CT058, CT144, CT172, and pbpB). Amplicons were sequenced and phylogenetic analysis was conducted.Results: Seven strains were detected in the eight infection episodes (in one patient, an identical strain being found in two episodes). Analysis of organotropism-related genes showed that these strains shared genetic features characteristic of genitotropic genotypes but not of trachoma strains. Three strains of genotype A showed a unique and new MLST-sequence type (ST551, allele profile 8-8-2-27-69). The four strains of genotype B belonged to ST138.Conclusions: C. trachomatis ompA genotypes A and B associated with trachoma, but detected sporadically in urogenital samples in trachoma-free countries, may be the result of recombination between strains adapted to trachoma and strains adapted to sexual transmission.
Ämnesord
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Mikrobiologi inom det medicinska området (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Microbiology in the medical area (hsv//eng)
- NATURVETENSKAP -- Biologi -- Mikrobiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Microbiology (hsv//eng)
Nyckelord
- Chlamydia trachomatis
- Multilocus sequence typing
- ompA genotypes A and B
- Organotropic genes
- Urogenital samples
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
Hitta via bibliotek
Till lärosätets databas