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WFRF:(J. Talbot Nicholas.)
 

Sökning: WFRF:(J. Talbot Nicholas.) > The beta-1,3-glucan...

LIBRIS Formathandbok  (Information om MARC21)
FältnamnIndikatorerMetadata
00003288naa a2200409 4500
001oai:DiVA.org:kth-205503
003SwePub
008170510s2017 | |||||||||||000 ||eng|
0247 a https://urn.kb.se/resolve?urn=urn:nbn:se:kth:diva-2055032 URI
0247 a https://doi.org/10.1111/cmi.126592 DOI
040 a (SwePub)kth
041 a engb eng
042 9 SwePub
072 7a ref2 swepub-contenttype
072 7a art2 swepub-publicationtype
1001 a Samalova, Marketa4 aut
2451 0a The beta-1,3-glucanosyltransferases (Gels) affect the structure of the rice blast fungal cell wall during appressorium-mediated plant infection
264 c 2016-10-11
264 1b Wiley-VCH Verlagsgesellschaft,c 2017
338 a print2 rdacarrier
500 a QC 20170510
520 a The fungal wall is pivotal for cell shape and function, and in interfacial protection during host infection and environmental challenge. Here, we provide the first description of the carbohydrate composition and structure of the cell wall of the rice blast fungus Magnaporthe oryzae. We focus on the family of glucan elongation proteins (Gels) and characterize five putative beta-1,3-glucan glucanosyltransferases that each carry the Glycoside Hydrolase 72 signature. We generated targeted deletion mutants of all Gel isoforms, that is, the GH72(+), which carry a putative carbohydrate-binding module, and the GH72(-)Gels, without this motif. We reveal that M. oryzae GH72(+) GELs are expressed in spores and during both infective and vegetative growth, but each individual Gel enzymes are dispensable for pathogenicity. Further, we demonstrated that Delta gel1 Delta gel3 Delta gel4 null mutant has a modified cell wall in which 1,3-glucans have a higher degree of polymerization and are less branched than the wild-type strain. The mutant showed significant differences in global patterns of gene expression, a hyper-branching phenotype and no sporulation, and thus was unable to cause rice blast lesions (except via wounded tissues). We conclude that Gel proteins play significant roles in structural modification of the fungal cell wall during appressorium-mediated plant infection.
650 7a MEDICIN OCH HÄLSOVETENSKAPx Medicinsk bioteknologix Medicinsk bioteknologi0 (SwePub)304012 hsv//swe
650 7a MEDICAL AND HEALTH SCIENCESx Medical Biotechnologyx Medical Biotechnology0 (SwePub)304012 hsv//eng
650 7a NATURVETENSKAPx Biologix Mikrobiologi0 (SwePub)106062 hsv//swe
650 7a NATURAL SCIENCESx Biological Sciencesx Microbiology0 (SwePub)106062 hsv//eng
7001 a Melida, Hugou KTH,Glykovetenskap4 aut0 (Swepub:kth)u140zccu
7001 a Vilaplana, Francisco,d 1979-u KTH,Glykovetenskap4 aut0 (Swepub:kth)u173a4yy
7001 a Bulone, Vincentu KTH,Glykovetenskap4 aut0 (Swepub:kth)u13o1auq
7001 a Soanes, Darren M.4 aut
7001 a Talbot, Nicholas J.4 aut
7001 a Gurr, Sarah J.4 aut
7102 a KTHb Glykovetenskap4 org
7730 t Cellular Microbiologyd : Wiley-VCH Verlagsgesellschaftg 19:3q 19:3x 1462-5814x 1462-5822
8564 u https://onlinelibrary.wiley.com/doi/pdfdirect/10.1111/cmi.12659
8564 8u https://urn.kb.se/resolve?urn=urn:nbn:se:kth:diva-205503
8564 8u https://doi.org/10.1111/cmi.12659

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