Global variability analysis of mRNA and protein concentrations across and within human tissues

Wegler, Christine (författare): Uppsala universitet,Institutionen för farmaci,DMPK, Research and Early Development Cardiovascular, Renal and Metabolism, BioPharmaceuticals R&D , AstraZeneca, Gothenburg SE-43183, Sweden

Wiśniewski, Jacek R (författare): Biochemical Proteomics Group, Department of Proteomics and Signal Transduction, Max Planck Institute of Biochemistry , Martinsried D-82152, Germany

Lundquist, Patrik (författare): Uppsala universitet,Institutionen för farmaci

Zettl, Katharina (författare): Biochemical Proteomics Group, Department of Proteomics and Signal Transduction, Max Planck Institute of Biochemistry , Martinsried D-82152, Germany

Åsberg, Anders (författare): Department of Pharmacy, University of Oslo , Oslo NO-0316, Norway; Department of Transplantation Medicine, Oslo University Hospital-Rikshospitalet , Oslo NO-0316, Norway

Hjelmesæth, Jøran (författare): Morbid Obesity Centre, Department of Medicine, Vestfold Hospital Trust , Tønsberg NO-3103, Norway; Department of Endocrinology, Morbid Obesity and Preventive Medicine, Institute of Clinical Medicine, University of Oslo , Oslo NO-0316, Norway

Andersson, Tommy B (författare): DMPK, Research and Early Development Cardiovascular, Renal and Metabolism, BioPharmaceuticals R&D , AstraZeneca, Gothenburg SE-43183, Sweden

Artursson, Per (författare): Uppsala universitet,Institutionen för farmaci,Science for Life Laboratory, SciLifeLab

(creator_code:org_t)

2019-10-30
2020
Engelska.
Ingår i: NAR Genomics and Bioinformatics. - : Oxford University Press (OUP). - 2631-9268. ; 2:1

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Abstract Ämnesord

Stäng

Genes and proteins show variable expression patterns throughout the human body. However, it is not clear whether relative differences in mRNA concentrations are retained on the protein level. Furthermore, inter-individual protein concentration variability within single tissue types has not been comprehensively explored. Here, we used the Gini index for in-depth concentration variability analysis of publicly available transcriptomics and proteomics data, and of an in-house proteomics dataset of human liver and jejunum from 38 donors. We found that the transfer of concentration variability from mRNA to protein is limited, that established ‘reference genes’ for data normalization vary markedly at the protein level, that protein concentrations cover a wide variability spectrum within single tissue types, and that concentration variability analysis can be a convenient starting point for identifying disease-associated proteins and novel biomarkers. Our results emphasize the importance of considering individual concentration levels, as opposed to population averages, for personalized systems biology analysis.

Av författaren/redakt...: Wegler, Christin ...; Ölander, Magnus; Wiśniewski, Jace ...; Lundquist, Patri ...; Zettl, Katharina; Åsberg, Anders; visa fler...; Hjelmesæth, Jøra ...; Andersson, Tommy ...; Artursson, Per; visa färre...

Om ämnet

MEDICIN OCH HÄLSOVETENSKAP: MEDICIN OCH HÄLS ...; och Medicinska och f ...; och Farmaceutiska ve ...

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