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  • Matuszewski, Damian J.Uppsala universitet,Avdelningen för visuell information och interaktion (author)

Learning Cell Nuclei Segmentation Using Labels Generated with Classical Image Analysis Methods

  • Article/chapterEnglish2021

Publisher, publication year, extent ...

  • University of West Bohemia,2021
  • printrdacarrier

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  • LIBRIS-ID:oai:DiVA.org:uu-453417
  • https://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-453417URI
  • https://doi.org/10.24132/CSRN.2021.3002.37DOI

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  • Language:English
  • Summary in:English

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  • Subject category:ref swepub-contenttype
  • Subject category:kon swepub-publicationtype

Notes

  • Creating manual annotations in a large number of images is a tedious bottleneck that limits deep learning use inmany applications. Here, we present a study in which we used the output of a classical image analysis pipeline aslabels when training a convolutional neural network (CNN). This may not only reduce the time experts spendannotating images but it may also lead to an improvement of results when compared to the output from the classicalpipeline used in training. In our application, i.e., cell nuclei segmentation, we generated the annotations usingCellProfiler (a tool for developing classical image analysis pipelines for biomedical applications) and trained onthem a U-Net-based CNN model. The best model achieved a 0.96 dice-coefficient of the segmented Nuclei and a0.84 object-wise Jaccard index which was better than the classical method used for generating the annotations by0.02 and 0.34, respectively. Our experimental results show that in this application, not only such training is feasiblebut also that the deep learning segmentations are a clear improvement compared to the output from the classicalpipeline used for generating the annotations.

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  • Ranefall, Petter,1968-Uppsala universitet,Avdelningen för visuell information och interaktion(Swepub:uu)peran517 (author)
  • Uppsala universitetAvdelningen för visuell information och interaktion (creator_code:org_t)

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  • In:Proceedings of the WSCG 2021: University of West Bohemia, s. 335-338

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