Search: WFRF:(Svensson Anna C.) >
Systematic evaluati...
Systematic evaluation of SARS-CoV-2 antigens enables a highly specific and sensitive multiplex serological COVID-19 assay
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- Hober, Sophia, Professor, 1965- (author)
- KTH Royal Institute of Technology
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- Hellström, Cecilia (author)
- KTH Royal Institute of Technology,KTH,Affinitets-proteomik,Science for Life Laboratory, SciLifeLab
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- Olofsson, Jennie (author)
- KTH Royal Institute of Technology,KTH,Science for Life Laboratory, SciLifeLab,Affinitets-proteomik
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show more...
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- Andersson, Eni (author)
- KTH Royal Institute of Technology,KTH,Science for Life Laboratory, SciLifeLab,Affinitets-proteomik
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- Bergström, Sofia (author)
- KTH Royal Institute of Technology,KTH,Science for Life Laboratory, SciLifeLab,Affinitets-proteomik
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- Jernbom Falk, August (author)
- KTH Royal Institute of Technology,KTH,Affinitets-proteomik,Science for Life Laboratory, SciLifeLab
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- Bayati, Shaghayegh (author)
- KTH Royal Institute of Technology,KTH,Div Affin Prote, Dept Prot Sci, SciLifeLab, Stockholm, Sweden.
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- Mravinacová, Sára (author)
- KTH Royal Institute of Technology,KTH,Proteinvetenskap
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- Sjöberg, Ronald (author)
- KTH Royal Institute of Technology,KTH,Proteinvetenskap
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- Yousef, Jamil (author)
- KTH Royal Institute of Technology,KTH,Proteinvetenskap
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- Skoglund, Lovisa (author)
- Karolinska Institutet,KTH Royal Institute of Technology,KTH,Proteinvetenskap
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- Kanje, Sara (author)
- KTH Royal Institute of Technology,KTH,Proteinvetenskap
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- Berling, Anna (author)
- KTH Royal Institute of Technology,KTH,Proteinvetenskap
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- Svensson, Anne-Sophie (author)
- KTH Royal Institute of Technology,KTH,Proteinvetenskap
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- Jensen, Gabriella (author)
- KTH Royal Institute of Technology,KTH,Proteinvetenskap
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- Enstedt, Henric (author)
- KTH Royal Institute of Technology,KTH,Proteinteknologi
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- Afshari, Delaram (author)
- KTH Royal Institute of Technology,KTH,Proteinteknologi
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- Xu, Lan Lan (author)
- KTH Royal Institute of Technology,KTH,Proteinvetenskap
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- Zwahlen, Martin (author)
- KTH Royal Institute of Technology,KTH,Science for Life Laboratory, SciLifeLab
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- von Feilitzen, Kalle (author)
- KTH Royal Institute of Technology,KTH,Systembiologi,Proteinteknologi,Science for Life Laboratory, SciLifeLab
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- Hanke, L. (author)
- Karolinska Institutet
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- Murrell, B. (author)
- Karolinska Institutet
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- McInerney, G. (author)
- Karolinska Institutet
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- Hedestam, G. B. K. (author)
- Karolinska Institutet
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- Lendel, Christofer (author)
- KTH Royal Institute of Technology,KTH,Tillämpad fysikalisk kemi
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- Roth, Robert G. (author)
- AstraZeneca, Sweden
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- Skoog, Ingmar, 1954 (author)
- University of Gothenburg,Gothenburg University,Göteborgs universitet,Centrum för åldrande och hälsa (AgeCap),Centre for Ageing and Health (Agecap)
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- Svenungsson, E. (author)
- Karolinska Institutet
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- Olsson, T. (author)
- Karolinska Institutet,Karolinska Institute
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- Fogdell-Hahn, A. (author)
- Karolinska Institutet,Karolinska Institute
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- Lindroth, Ylva (author)
- Lund University,Lunds universitet,Avdelningen för medicinsk mikrobiologi,Institutionen för laboratoriemedicin,Medicinska fakulteten,Division of Medical Microbiology,Department of Laboratory Medicine,Faculty of Medicine
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- Lundgren, Maria (author)
- Region Skåne
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- Maleki, K. T. (author)
- Karolinska Institutet,Karolinska Institute,Public Health Agency of Sweden
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- Lagerqvist, N. (author)
- Public Health Agency of Sweden
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- Klingstrom, J. (author)
- Karolinska Institutet,Karolinska Institute,Public Health Agency of Sweden
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- Rodrigues, R. D. (author)
- Karolinska University Hospital
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- Muschiol, S. (author)
- Karolinska Institutet
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Bogdanovic, G. (author)
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- Sivertsson, Åsa (author)
- KTH Royal Institute of Technology,KTH,Science for Life Laboratory, SciLifeLab,Systembiologi
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- Eklund, Carina (author)
- Karolinska Institutet
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- Lagheden, Camilla (author)
- Karolinska Institutet
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- Dillner, Joakim (author)
- Karolinska Institutet
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Sivertsson, A. (author)
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- Havervall, S. (author)
- Karolinska Institutet
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- Thalin, C. (author)
- Karolinska Institutet
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- Tegel, Hanna (author)
- KTH Royal Institute of Technology,KTH,Proteinvetenskap
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- Pin, Elisa (author)
- KTH Royal Institute of Technology,KTH,Affinitets-proteomik,Science for Life Laboratory, SciLifeLab
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- Månberg, Anna, 1985- (author)
- KTH Royal Institute of Technology,KTH,Science for Life Laboratory, SciLifeLab,Affinitets-proteomik
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- Hedhammar, My, Professor, 1975- (author)
- KTH Royal Institute of Technology,KTH,Centrum för Bioprocessteknik, CBioPT,Proteinteknologi,Science for Life Laboratory, SciLifeLab
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- Nilsson, Peter (author)
- KTH Royal Institute of Technology,KTH,Science for Life Laboratory, SciLifeLab,Affinitets-proteomik
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- Arroyo Mühr, Laila Sara (author)
- Karolinska Institute
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(creator_code:org_t)
- 2021-07-19
- 2021
- English.
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In: Clinical & Translational Immunology. - : Wiley. - 2050-0068. ; 10:7
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http://dx.doi.org/10... (free)
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https://gup.ub.gu.se...
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https://doi.org/10.1...
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https://urn.kb.se/re...
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Abstract
Subject headings
Close
- Objective. The COVID-19 pandemic poses an immense need for accurate, sensitive and high-throughput clinical tests, and serological assays are needed for both overarching epidemiological studies and evaluating vaccines. Here, we present the development and validation of a high-throughput multiplex bead-based serological assay. Methods. More than 100 representations of SARS-CoV-2 proteins were included for initial evaluation, including antigens produced in bacterial and mammalian hosts as well as synthetic peptides. The five best-performing antigens, three representing the spike glycoprotein and two representing the nucleocapsid protein, were further evaluated for detection of IgG antibodies in samples from 331 COVID-19 patients and convalescents, and in 2090 negative controls sampled before 2020. Results. Three antigens were finally selected, represented by a soluble trimeric form and the S1-domain of the spike glycoprotein as well as by the C-terminal domain of the nucleocapsid. The sensitivity for these three antigens individually was found to be 99.7%, 99.1% and 99.7%, and the specificity was found to be 98.1%, 98.7% and 95.7%. The best assay performance was although achieved when utilising two antigens in combination, enabling a sensitivity of up to 99.7% combined with a specificity of 100%. Requiring any two of the three antigens resulted in a sensitivity of 99.7% and a specificity of 99.4%. Conclusion. These observations demonstrate that a serological test based on a combination of several SARS-CoV-2 antigens enables a highly specific and sensitive multiplex serological COVID-19 assay.
Subject headings
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Immunologi inom det medicinska området (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Immunology in the medical area (hsv//eng)
- MEDICIN OCH HÄLSOVETENSKAP -- Klinisk medicin -- Infektionsmedicin (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Clinical Medicine -- Infectious Medicine (hsv//eng)
Keyword
- COVID-19
- IgG
- multiplex
- SARS-CoV-2
- serological assay
- coronavirus
- protein
- spike
- Immunology
- COVID-19
Publication and Content Type
- ref (subject category)
- art (subject category)
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- By the author/editor
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Hober, Sophia, P ...
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Hellström, Cecil ...
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Olofsson, Jennie
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Andersson, Eni
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Bergström, Sofia
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Jernbom Falk, Au ...
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show more...
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Bayati, Shaghaye ...
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Mravinacová, Sár ...
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Sjöberg, Ronald
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Yousef, Jamil
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Skoglund, Lovisa
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Kanje, Sara
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Berling, Anna
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Svensson, Anne-S ...
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Jensen, Gabriell ...
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Enstedt, Henric
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Afshari, Delaram
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Xu, Lan Lan
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Zwahlen, Martin
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von Feilitzen, K ...
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Hanke, L.
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Murrell, B.
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McInerney, G.
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Hedestam, G. B. ...
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Lendel, Christof ...
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Roth, Robert G.
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Skoog, Ingmar, 1 ...
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Svenungsson, E.
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Olsson, T.
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Fogdell-Hahn, A.
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Lindroth, Ylva
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Lundgren, Maria
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Maleki, K. T.
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Lagerqvist, N.
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Klingstrom, J.
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Rodrigues, R. D.
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Muschiol, S.
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Bogdanovic, G.
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Sivertsson, Åsa
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Eklund, Carina
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Lagheden, Camill ...
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Dillner, Joakim
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Sivertsson, A.
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Havervall, S.
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Thalin, C.
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Tegel, Hanna
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Pin, Elisa
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Månberg, Anna, 1 ...
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Hedhammar, My, P ...
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Nilsson, Peter
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Arroyo Mühr, Lai ...
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- About the subject
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- MEDICAL AND HEALTH SCIENCES
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MEDICAL AND HEAL ...
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and Basic Medicine
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and Immunology in th ...
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- MEDICAL AND HEALTH SCIENCES
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MEDICAL AND HEAL ...
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and Clinical Medicin ...
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and Infectious Medic ...
- Articles in the publication
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Clinical & Trans ...
- By the university
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University of Gothenburg
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Royal Institute of Technology
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Lund University
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Karolinska Institutet