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Amperometric Biosen...
Amperometric Biosensor for estimation of Glucose-6-phosphate Using Prussian Blue Nanoparticles.
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- Banerjee, S (författare)
- Department of Polymer Science and Technology, University of Calcutta, India
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- Sarkar, Priya (författare)
- Department of Polymer Science and Technology, University of Calcutta, India
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- Turner, Anthony, 1950- (författare)
- Linköpings universitet,Biosensorer och bioelektronik,Tekniska högskolan,Biosensors & Bioelectronics
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(creator_code:org_t)
- Elsevier BV, 2013
- 2013
- Engelska.
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Ingår i: Analytical Biochemistry. - : Elsevier BV. - 0003-2697 .- 1096-0309. ; 439:2, s. 194-200
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https://urn.kb.se/re...
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https://doi.org/10.1...
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Abstract
Ämnesord
Stäng
- Glucose-6-phosphateplays an important role in carbohydrate metabolism of all living organisms.Compared to the conventional analytical methods available for estimation of glucose-6-phosphate,the biosensors having relative simplicity, specificity, low-cost and fastresponse time are a promising alternative. We have reported a glucose-6-phosphatesensor based on screen-printed electrode utilizing Prussian blue nanoparticlesand enzymes, glucose-6-phosphate dehydrogenase and glutathione reductase. The Prussianblue nanoparticles acted as a mediator enhancing the rate of electrochemical responses.The Fourier transforminfrared spectroscopy and energy-dispersiveX-ray spectroscopy study confirmed the formation of Prussian blue, whereas, the atomic forced microscopy revealed that Prussian bluenanoparticles were about 25-30 nm in diameter. To obtainmaximum amperometric response, optimization studies were conducted for pH,enzyme and cofactor loading. The proposed glucose-6-phosphate biosensor showed goodstability, rapid response time and broad linear response in the range of 0.01-1.25mM and detection limit of 6.3 mM. The biosensor also worked well for serum samples and exhibitedexcellent anti-interference ability.
Nyckelord
- Glucose-6-phosphate; Prussian blue nanoparticles; Screen-printed electrode; Amperometry
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
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