WFRF:(Wang Shujiang)
Sökning: WFRF:(Wang Shujiang) > Insights into dynam...
- Wang, Shujiang,1985-Uppsala universitet,Polymerkemi (författare)
Insights into dynamic covalent chemistry for bioconjugation applications
- BokEngelska2017
Förlag, utgivningsår, omfång ...
- Uppsala :Acta Universitatis Upsaliensis,2017
- 59 s.
- electronicrdacarrier
Nummerbeteckningar
- LIBRIS-ID:oai:DiVA.org:uu-329022
- ISBN:9789151300658
- https://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-329022URI
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- Språk:engelska
- Sammanfattning på:engelska
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Klassifikation
Serie
- Digital Comprehensive Summaries of Uppsala Dissertations from the Faculty of Science and Technology,1651-6214 ;1554
Anmärkningar
- Dynamic covalent chemistry (DCC) is currently exploited in several areas of biomedical applications such as in drug discovery, sensing, molecular separation, catalysis etc. Hydrazone and oxime chemistry have several advantages, such as mild reaction conditions, selectivity, efficiency, and biocompatibility and therefore, have the potential to be for bioconjugation applications. However, these reactions suffer from major drawbacks of slow reaction rate and poor bond stability under physiological conditions. In this regard, the work presented in this thesis focuses on designing novel bioconjugation reactions amenable under physiological conditions with tunable reaction kinetics and conjugation stability.The first part of the thesis presents different strategies of dynamic covalent reactions utilized for biomedical applications. In the next part, a detailed study related to the mechanism and catalysis of oxime chemistry was investigated in the presence of various catalysts. Aniline, carboxylate and saline were selective as target catalysts and their reaction kinetics were compared under physiological conditions (Paper I and II). Then we attempted to explore the potential of those chemistries in fabricating 3D hydrogel scaffolds for regenerative medicine application. A novel mild and regioselective method was devised to introduce an aldehyde moiety onto glycosaminoglycans structure. This involved the introduction of amino glycerol to glycosaminoglycans, followed by regioselective oxidation of tailed flexible diol without affecting the C2-C3 diol groups on the disaccharide repeating unit. The oxidation rate of the tailed flexible diol was 4-times faster than that of C2-C3 diol groups of native glycosaminoglycan. This strategy preserves the structural integrity of the glycosaminoglycans and provides a functional aldehyde moiety (Paper III). Further, different types of hydrazones were designed and their hydrolytic stability under acidic condition was carefully evaluated. The hydrazone linkage with the highest hydrolytic stability was utilized in the preparation of extracellular matrix hydrogel for delivery of bone morphogenetic proteins 2 in bone regeneration (Paper IV) and studied for controlled release of the growth factor (Paper III).In summary, this thesis presents a selection of strategies for designing bioconjugation chemistries that possess tunable stability and reaction kinetics under physiological conditions. These chemistries are powerful tools for conjugation of biomolecules for the biomedical applications.
Ämnesord och genrebeteckningar
- NATURVETENSKAP Kemi Polymerkemi hsv//swe
- NATURAL SCIENCES Chemical Sciences Polymer Chemistry hsv//eng
- NATURVETENSKAP Kemi Materialkemi hsv//swe
- NATURAL SCIENCES Chemical Sciences Materials Chemistry hsv//eng
- NATURVETENSKAP Kemi Organisk kemi hsv//swe
- NATURAL SCIENCES Chemical Sciences Organic Chemistry hsv//eng
- dynamic covalent chemistry
- reaction mechanism
- hydrogel
- bioconjugation
- catalysis
- Kemi med inriktning mot polymerkemi
- Chemistry with specialization in Polymer Chemistry
Biuppslag (personer, institutioner, konferenser, titlar ...)
- Varghese, Oommen,Associate professorUppsala universitet,Polymerkemi (preses)
- Tirelli, Nicola,ProfessorThe University of Manchester (opponent)
- Uppsala universitetPolymerkemi (creator_code:org_t)
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