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  • Fransson, ThomasKTH Royal Institute of Technology (författare)

Effects of x-ray free-electron laser pulse intensity on the Mn K β 1,3x-ray emission spectrum in photosystem II - A case study for metalloprotein crystals and solutions

  • Artikel/kapitelEngelska2021

Förlag, utgivningsår, omfång ...

  • American Institute of Physics (AIP),2021
  • electronicrdacarrier

Nummerbeteckningar

  • LIBRIS-ID:oai:DiVA.org:umu-190115
  • https://urn.kb.se/resolve?urn=urn:nbn:se:umu:diva-190115URI
  • https://doi.org/10.1063/4.0000130DOI
  • https://lup.lub.lu.se/record/a5416ce3-bd02-4b87-a78f-df4ceeaad078URI

Kompletterande språkuppgifter

  • Språk:engelska
  • Sammanfattning på:engelska

Ingår i deldatabas

Klassifikation

  • Ämneskategori:ref swepub-contenttype
  • Ämneskategori:art swepub-publicationtype

Anmärkningar

  • In the last ten years, x-ray free-electron lasers (XFELs) have been successfully employed to characterize metalloproteins at room temperature using various techniques including x-ray diffraction, scattering, and spectroscopy. The approach has been to outrun the radiation damage by using femtosecond (fs) x-ray pulses. An example of an important and damage sensitive active metal center is the Mn4CaO5 cluster in photosystem II (PS II), the catalytic site of photosynthetic water oxidation. The combination of serial femtosecond x-ray crystallography and Kβ x-ray emission spectroscopy (XES) has proven to be a powerful multimodal approach for simultaneously probing the overall protein structure and the electronic state of the Mn4CaO5 cluster throughout the catalytic (Kok) cycle. As the observed spectral changes in the Mn4CaO5 cluster are very subtle, it is critical to consider the potential effects of the intense XFEL pulses on the Kβ XES signal. We report here a systematic study of the effects of XFEL peak power, beam focus, and dose on the Mn Kβ1,3 XES spectra in PS II over a wide range of pulse parameters collected over seven different experimental runs using both microcrystal and solution PS II samples. Our findings show that for beam intensities ranging from ∼5 × 1015 to 5 × 1017 W/cm2 at a pulse length of ∼35 fs, the spectral effects are small compared to those observed between S-states in the Kok cycle. Our results provide a benchmark for other XFEL-based XES studies on metalloproteins, confirming the viability of this approach.

Ämnesord och genrebeteckningar

Biuppslag (personer, institutioner, konferenser, titlar ...)

  • Alonso-Mori, RobertoLinac Coherent Light Source, SLAC National Accelerator Laboratory, CA, Menlo Park, United States,Stanford Linear Accelerator Center (SLAC) (författare)
  • Chatterjee, RuchiraMolecular Biophysics and Integrated Bioimaging Division, Lawrence Berkeley National Laboratory, CA, Berkeley, United States (författare)
  • Cheah, Mun HonUppsala University (författare)
  • Ibrahim, MohamedHumboldt-Universität zu Berlin, Department of Biology, Berlin, Germany (författare)
  • Hussein, RanaHumboldt-Universität zu Berlin, Department of Biology, Berlin, Germany (författare)
  • Zhang, MiaoHumboldt-Universität zu Berlin, Department of Biology, Berlin, Germany (författare)
  • Fuller, FranklinLinac Coherent Light Source, SLAC National Accelerator Laboratory, CA, Menlo Park, United States,Stanford Linear Accelerator Center (SLAC) (författare)
  • Gul, SherazMolecular Biophysics and Integrated Bioimaging Division, Lawrence Berkeley National Laboratory, CA, Berkeley, United States (författare)
  • Kim, In-SikMolecular Biophysics and Integrated Bioimaging Division, Lawrence Berkeley National Laboratory, CA, Berkeley, United States (författare)
  • Simon, Philipp S.Molecular Biophysics and Integrated Bioimaging Division, Lawrence Berkeley National Laboratory, CA, Berkeley, United States,Stanford Linear Accelerator Center (SLAC) (författare)
  • Bogacz, IsabelMolecular Biophysics and Integrated Bioimaging Division, Lawrence Berkeley National Laboratory, CA, Berkeley, United States (författare)
  • Makita, HirokiMolecular Biophysics and Integrated Bioimaging Division, Lawrence Berkeley National Laboratory, CA, Berkeley, United States (författare)
  • de Lichtenberg, CasperUppsala University,Umeå University,Umeå universitet,Kemiska institutionen,Department of Chemistry - Ångström Laboratory, Molecular Biomimetics, Uppsala University, Uppsala, Sweden; Section of Biomolecular Sciences, Department of Biology, University of Copenhagen, Copenhagen N, Denmark(Swepub:umu)cade0029 (författare)
  • Song, SanghoonLinac Coherent Light Source, SLAC National Accelerator Laboratory, CA, Menlo Park, United States (författare)
  • Batyuk, AlexanderLinac Coherent Light Source, SLAC National Accelerator Laboratory, CA, Menlo Park, United States,Stanford Linear Accelerator Center (SLAC) (författare)
  • Sokaras, DimosthenisStanford Synchrotron Radiation Lightsource, SLAC National Accelerator Laboratory, CA, Menlo Park, United States,Stanford Linear Accelerator Center (SLAC) (författare)
  • Massad, RamziMolecular Biophysics and Integrated Bioimaging Division, Lawrence Berkeley National Laboratory, CA, Berkeley, United States (författare)
  • Doyle, MargaretMolecular Biophysics and Integrated Bioimaging Division, Lawrence Berkeley National Laboratory, CA, Berkeley, United States (författare)
  • Britz, AlexanderLinac Coherent Light Source, SLAC National Accelerator Laboratory, CA, Menlo Park, United States; Stanford PULSE Institute, SLAC National Accelerator Laboratory, CA, Menlo Park, United States,Stanford Linear Accelerator Center (SLAC) (författare)
  • Weninger, ClemensLund University,Lunds universitet,MAX IV-laboratoriet,MAX IV Laboratory(Swepub:lu)cl1816we (författare)
  • Zouni, AthinaHumboldt-Universität zu Berlin, Department of Biology, Berlin, Germany (författare)
  • Messinger, Johannes,1963-Uppsala University,Umeå University,Umeå universitet,Kemiska institutionen,Department of Chemistry - Ångström Laboratory, Molecular Biomimetics, Uppsala University, Uppsala, Sweden(Swepub:umu)jome0007 (författare)
  • Yachandra, Vittal K.Molecular Biophysics and Integrated Bioimaging Division, Lawrence Berkeley National Laboratory, CA, Berkeley, United States (författare)
  • Yano, JunkoMolecular Biophysics and Integrated Bioimaging Division, Lawrence Berkeley National Laboratory, CA, Berkeley, United States (författare)
  • Kern, JanMolecular Biophysics and Integrated Bioimaging Division, Lawrence Berkeley National Laboratory, CA, Berkeley, United States (författare)
  • Bergmann, UweDepartment of Physics, University of Wisconsin-Madison, WI, Madison, United States (författare)
  • KTH Royal Institute of TechnologyLinac Coherent Light Source, SLAC National Accelerator Laboratory, CA, Menlo Park, United States (creator_code:org_t)

Sammanhörande titlar

  • Ingår i:Structural Dynamics: American Institute of Physics (AIP)8:62329-7778

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