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Structural basis fo...
Structural basis for synthesis of inflammatory mediators by human leukotriene C4 synthase
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- Niegowski, Damian, 1978- (author)
- Stockholms universitet,Institutionen för biokemi och biofysik,Pär Nordlund,Strukturbiokemi
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- Martinez, Daniel (author)
- Stockholms universitet,Institutionen för biokemi och biofysik,Strukturbiokemi
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Wetterholm, Anders (author)
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Kohl, Andreas (author)
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McCarthy, Andrew (author)
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Ohlson, Eva (author)
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- Hammarberg, Tove (author)
- Karolinska Institutet
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- Häggström, Jesper (author)
- Karolinska Institutet
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Nordlund, Pär (author)
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- Eshaghi, S (author)
- Karolinska Institutet
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(creator_code:org_t)
- 2007-07-15
- 2007
- English.
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In: Nature. - : Springer Science and Business Media LLC. - 0028-0836 .- 1476-4687. ; 448:7153, s. 613-616
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Abstract
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- Cysteinyl leukotrienes are key mediators in inflammation and have an important role in acute and chronic inflammatory diseases of the cardiovascular and respiratory systems, in particular bronchial asthma. In the biosynthesis of cysteinyl leukotrienes, conversion of arachidonic acid forms the unstable epoxide leukotriene A4 (LTA4). This intermediate is conjugated with glutathione (GSH) to produce leukotriene C4 (LTC4) in a reaction catalysed by LTC4 synthase1: this eaction is the key step in cysteinyl leukotriene formation. Here we present the rystal structure of the human LTC4 synthase in its apo and GSH-complexed forms to 2.00 and 2.15 A ̊resolution, respectively. The structure reveals a homotrimer, here each monomer is composed of four transmembrane segments. The structure of the enzyme in complex with substrate reveals that the active site enforces a orseshoe-shaped conformation on GSH, and effectively positions the thiol group or activation by a nearby arginine at the membrane–enzyme interface. In addition, the structure provides a model for how the v-end of the lipophilic co-substrate is pinned at one end of a hydrophobic cleft, providing a molecular ‘ruler’ to align the eactive epoxide at the thiol of glutathione. This provides new structural insights nto the mechanism of LTC4 formation, and also suggests that the observed inding and activation of GSH might be common for a family of homologous proteins mportant for inflammatory and detoxification responses.
Subject headings
- NATURVETENSKAP -- Biologi -- Strukturbiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Structural Biology (hsv//eng)
Keyword
- ltc4
- leukotriene synthase
- lipid binding
- cysteinyl leokotrienes
- NATURAL SCIENCES
- NATURVETENSKAP
- strukturbiologi
- Structural Biology
- Structural biology
- Biochemistry
Publication and Content Type
- ref (subject category)
- art (subject category)
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- By the author/editor
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Niegowski, Damia ...
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Martinez, Daniel
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Wetterholm, Ande ...
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Kohl, Andreas
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McCarthy, Andrew
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Ohlson, Eva
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show more...
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Hammarberg, Tove
-
Häggström, Jespe ...
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Nordlund, Pär
-
Eshaghi, S
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show less...
- About the subject
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- NATURAL SCIENCES
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NATURAL SCIENCES
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and Biological Scien ...
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and Structural Biolo ...
- Articles in the publication
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Nature
- By the university
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Stockholm University
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Karolinska Institutet