Structural evidence for the evolution of xyloglucanase activity from xyloglucan endo-transglycosylases: Biological implications for cell wall metabolism

Sökning: L773:1040 4651 > Structural evidence...

Structural evidence for the evolution of xyloglucanase activity from xyloglucan endo-transglycosylases : Biological implications for cell wall metabolism

QC 20100525
High-resolution, three-dimensional structures of the archetypal glycoside hydrolase family 16 (GH16) endo-xyloglucanases Tm-NXG1 and Tm-NXG2 from nasturtium (Tropaeolum majus) have been solved by x-ray crystallography. Key structural features that modulate the relative rates of substrate hydrolysis to transglycosylation in the GH16 xyloglucan-active enzymes were identified by structure-function studies of the recombinantly expressed enzymes in comparison with data for the strict xyloglucan endo-transglycosylase Ptt-XET16-34 from hybrid aspen ( Populus tremula 3 Populus tremuloides). Production of the loop deletion variant Tm-NXG1-Delta YNIIG yielded an enzyme that was structurally similar to Ptt- XET16-34 and had a greatly increased transglycosylation: hydrolysis ratio. Comprehensive bioinformatic analyses of XTH gene products, together with detailed kinetic data, strongly suggest that xyloglucanase activity has evolved as a gain of function in an ancestral GH16 XET to meet specific biological requirements during seed germination, fruit ripening, and rapid wall expansion.

NATURVETENSKAP Biologi Biokemi och molekylärbiologi hsv//swe
NATURAL SCIENCES Biological Sciences Biochemistry and Molecular Biology hsv//eng
tropaeolum-majus l
germinated nasturtium seeds
glycoside hydrolases
expression analysis
kappa-carrageenase
sequence alignment
crystal-structures
pichia-pastoris
hybrid aspen
endotransglycosylase
Biochemistry
Biokemi

Eklöf, Jens M.KTH,Glykovetenskap(Swepub:kth)u15pz720 (författare)
Michel, Gurvan (författare)
Kallas, Åsa M.KTH,Skolan för bioteknologi (BIO)(Swepub:kth)u1335wv6 (författare)
Teeri, Tuula T.KTH,Glykovetenskap(Swepub:kth)u1vcejiv (författare)
Czjzek, Mirjam (författare)
Brumer, HarryKTH,Glykovetenskap(Swepub:kth)u1e8oacn (författare)
KTHSkolan för bioteknologi (BIO) (creator_code:org_t)

Ingår i:The Plant Cell: Oxford University Press (OUP)19:6, s. 1947-19631040-46511532-298X

Av författaren/redakt...: Baumann, Martin ...; Eklöf, Jens M.; Michel, Gurvan; Kallas, Åsa M.; Teeri, Tuula T.; Czjzek, Mirjam; visa fler...; Brumer, Harry; visa färre...

Om ämnet

Kungliga biblioteket hanterar dina personuppgifter i enlighet med EU:s dataskyddsförordning (2018), GDPR. Läs mer om hur det funkar här.
Så här hanterar KB dina uppgifter vid användning av denna tjänst.