Structural evidence for the evolution of xyloglucanase activity from xyloglucan endo-transglycosylases: Biological implications for cell wall metabolism

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Fältnamn	Indikatorer	Metadata
000		03342naa a2200529 4500
001		oai:DiVA.org:kth-16832
003		SwePub
008		100805s2007 \| \|\|\|\|\|\|\|\|\|\|\|000 \|\|eng\|
024	7	a https://urn.kb.se/resolve?urn=urn:nbn:se:kth:diva-168322 URI
024	7	a https://doi.org/10.1105/tpc.107.0513912 DOI
040		a (SwePub)kth
041		a engb eng
042		9 SwePub
072	7	a ref2 swepub-contenttype
072	7	a art2 swepub-publicationtype
100	1	a Baumann, Martin J.u KTH,Skolan för bioteknologi (BIO)4 aut0 (Swepub:kth)u12yxgbu
245	1 0	a Structural evidence for the evolution of xyloglucanase activity from xyloglucan endo-transglycosylases :b Biological implications for cell wall metabolism
264		c 2007-06-08
264	1	b Oxford University Press (OUP),c 2007
338		a print2 rdacarrier
500		a QC 20100525
520		a High-resolution, three-dimensional structures of the archetypal glycoside hydrolase family 16 (GH16) endo-xyloglucanases Tm-NXG1 and Tm-NXG2 from nasturtium (Tropaeolum majus) have been solved by x-ray crystallography. Key structural features that modulate the relative rates of substrate hydrolysis to transglycosylation in the GH16 xyloglucan-active enzymes were identified by structure-function studies of the recombinantly expressed enzymes in comparison with data for the strict xyloglucan endo-transglycosylase Ptt-XET16-34 from hybrid aspen ( Populus tremula 3 Populus tremuloides). Production of the loop deletion variant Tm-NXG1-Delta YNIIG yielded an enzyme that was structurally similar to Ptt- XET16-34 and had a greatly increased transglycosylation: hydrolysis ratio. Comprehensive bioinformatic analyses of XTH gene products, together with detailed kinetic data, strongly suggest that xyloglucanase activity has evolved as a gain of function in an ancestral GH16 XET to meet specific biological requirements during seed germination, fruit ripening, and rapid wall expansion.
650	7	a NATURVETENSKAPx Biologix Biokemi och molekylärbiologi0 (SwePub)106022 hsv//swe
650	7	a NATURAL SCIENCESx Biological Sciencesx Biochemistry and Molecular Biology0 (SwePub)106022 hsv//eng
653		a tropaeolum-majus l
653		a germinated nasturtium seeds
653		a glycoside hydrolases
653		a expression analysis
653		a kappa-carrageenase
653		a sequence alignment
653		a crystal-structures
653		a pichia-pastoris
653		a hybrid aspen
653		a endotransglycosylase
653		a Biochemistry
653		a Biokemi
700	1	a Eklöf, Jens M.u KTH,Glykovetenskap4 aut0 (Swepub:kth)u15pz720
700	1	a Michel, Gurvan4 aut
700	1	a Kallas, Åsa M.u KTH,Skolan för bioteknologi (BIO)4 aut0 (Swepub:kth)u1335wv6
700	1	a Teeri, Tuula T.u KTH,Glykovetenskap4 aut0 (Swepub:kth)u1vcejiv
700	1	a Czjzek, Mirjam4 aut
700	1	a Brumer, Harryu KTH,Glykovetenskap4 aut0 (Swepub:kth)u1e8oacn
710	2	a KTHb Skolan för bioteknologi (BIO)4 org
773	0	t The Plant Celld : Oxford University Press (OUP)g 19:6, s. 1947-1963q 19:6<1947-1963x 1040-4651x 1532-298X
856	4	u http://www.plantcell.org/content/19/6/1947.full.pdf
856	4 8	u https://urn.kb.se/resolve?urn=urn:nbn:se:kth:diva-16832
856	4 8	u https://doi.org/10.1105/tpc.107.051391

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Av författaren/redakt...: Baumann, Martin ...; Eklöf, Jens M.; Michel, Gurvan; Kallas, Åsa M.; Teeri, Tuula T.; Czjzek, Mirjam; visa fler...; Brumer, Harry; visa färre...

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