Immunohistochemical and histochemical findings favoring the occurrence of autocrine/paracrine as well as nerve-related cholinergic effects in chronic painful patellar tendon tendinosis.

↓ Direkt till sidans innehåll
↓ Direkt till sidans sekundära innehåll (sidomenyn)

Sökning: L773:1059 910X OR L773:1097 0029 > Immunohistochemical...

Danielson, PatrikUmeå universitet,Anatomi (författare)

Immunohistochemical and histochemical findings favoring the occurrence of autocrine/paracrine as well as nerve-related cholinergic effects in chronic painful patellar tendon tendinosis.

Artikel/kapitelEngelska2006

Förlag, utgivningsår, omfång ...

2006-07-07
Wiley,2006
printrdacarrier

Nummerbeteckningar

LIBRIS-ID:oai:DiVA.org:umu-12694
https://urn.kb.se/resolve?urn=urn:nbn:se:umu:diva-12694URI
https://doi.org/10.1002/jemt.20351DOI

Kompletterande språkuppgifter

Språk:engelska
Sammanfattning på:engelska

Ingår i deldatabas

SwePubSwePub

Klassifikation

Ämneskategori:ref swepub-contenttype
Ämneskategori:art swepub-publicationtype

Anmärkningar

The pathogenesis of the pain in patellar tendon tendinosis ("jumper's knee") is unclear. We have recently presented new information about the sensory nervous system in the human patellar tendon, but there is very little information regarding the possible occurrence of a cholinergic system in this tendon. In the present study, specimens of pain-free normal tendons and chronically painful tendinosis tendons were examined by different immunohistochemical and histochemical methods. Antibodies against the M(2) receptor, choline acetyltransferase (ChAT), and vesicular acetylcholine transporter (VAChT) were applied, and staining for demonstration of activity of acetylcholinesterase (AChE) was also utilized. It was found that immunoreactions for the M(2) receptor could be detected intracellularly in both blood vessel cells and tenocytes, especially in tendinosis specimens. Furthermore, in the tendinosis specimens, some tenocytes were seen to exhibit immunoreaction for ChAT and VAChT. AChE reactions were seen in fine nerve fibers associated with small blood vessels in both the normal control tendons and the tendinosis tendons. The observations suggest that there is both a nerve related and a local cholinergic system in the human patellar tendon. As ChAT and VAChT immunoreactions were detected in tenocytes of tendinosis tendons, these cells might be a source of local acetylcholine (Ach) production. As both tenocytes and blood vessel cells were found to exhibit immunoreactions for the M(2) receptor, it is likely that both of these tissue cells may be influenced by ACh. Thus, in conclusion, there appears to be an upregulation of the cholinergic system, and an occurrence of autocrine/paracrine effects in this system, in the tendinosis patellar tendon.

Ämnesord och genrebeteckningar

Acetylcholine/biosynthesis
Acetylcholinesterase/analysis
Adult
Autocrine Communication
Blood Vessels/metabolism/pathology
Choline O-Acetyltransferase/analysis
Cholinergic Fibers/*chemistry/pathology
Chronic Disease
Female
Humans
Immunohistochemistry
Male
Middle Aged
Pain/pathology
Paracrine Communication
Patella/pathology
Receptor; Muscarinic M2/analysis
Tendinopathy/metabolism/*pathology
Vesicular Acetylcholine Transport Proteins/analysis

Biuppslag (personer, institutioner, konferenser, titlar ...)

Alfredson, HåkanUmeå universitet,Idrottsmedicin(Swepub:umu)haal0002 (författare)
Forsgren, StureUmeå universitet,Anatomi(Swepub:umu)stfo0001 (författare)
Umeå universitetAnatomi (creator_code:org_t)

Sammanhörande titlar

Ingår i:Microscopy research and technique (Print): Wiley69:10, s. 808-8191059-910X1097-0029

Internetlänk

Hitta via bibliotek

Microscopy research and technique (Print) (Sök värdpublikationen i LIBRIS)

Till lärosätets databas

Hitta mer i SwePub

Av författaren/redakt...: Danielson, Patri ...; Alfredson, Håkan; Forsgren, Sture

Artiklar i publikationen: Microscopy resea ...

Av lärosätet: Umeå universitet

Sök utanför SwePub

Sök vidare i:: Google; Google Book Search; Google Scholar

Kungliga biblioteket hanterar dina personuppgifter i enlighet med EU:s dataskyddsförordning (2018), GDPR. Läs mer om hur det funkar här.
Så här hanterar KB dina uppgifter vid användning av denna tjänst.

LIBRIS.kb.se