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Changes in insulin ...
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Bäck, KarolinaLinköpings universitet,Cellbiologi,Hälsouniversitetet
(författare)
Changes in insulin and IGF-I receptor expression during differentiation of human preadipocytes
- Artikel/kapitelEngelska2009
Förlag, utgivningsår, omfång ...
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Elsevier BV,2009
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electronicrdacarrier
Nummerbeteckningar
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LIBRIS-ID:oai:DiVA.org:liu-17895
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https://urn.kb.se/resolve?urn=urn:nbn:se:liu:diva-17895URI
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https://doi.org/10.1016/j.ghir.2008.06.004DOI
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Språk:engelska
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Sammanfattning på:engelska
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Ämneskategori:ref swepub-contenttype
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Ämneskategori:art swepub-publicationtype
Anmärkningar
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Original Publication: Karolina Bäck and Hans Arnqvist, Changes in insulin and IGF-I receptor expression during differentiation of human preadipocytes, 2009, GROWTH HORMONE and IGF RESEARCH, (19), 2, 101-111. http://dx.doi.org/10.1016/j.ghir.2008.06.004 Copyright: Elsevier Science B.V., Amsterdam http://www.elsevier.com/
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Mature adipocytes originate from fibroblast-like precursor cells, preadipocytes, which differentiate to obtain the characteristics of adipocytes. Our aim was to investigate how differentiation of human preadipocytes affects the distribution of insulin receptors (IR) and IGF-I receptors (IGF-IR) and other cell characteristics. Preadipocytes were differentiated using indomethacine, dexamethasone, isobutyl-methylxantine (IBMX) and high concentration of insulin. Gene expression was quantified by real-time RT-PCT in preadipocytes (PA), differentiated preadipocytes (dPA) and mature adipocytes (mAD). The amount of expressed receptor protein was analyzed using receptor specific ELISAs and Western blot. We also studied DNA synthesis with radiolabeled thymidine incorporation and glucose accumulation with radiolabeled glucose. Differentiation of PA increased gene expression of IR but not IGF-IR, GLUT4, growth hormone receptor (GHR) and adiponectin appeared or increased. In PA and dPA only IR-A was expressed whereas also IR-B was detected in mAD. By Western blot and ELISA, IR and IGF-IR was phosphorylated by their own ligant at 1 nM and in dPA the acitivation of both receptors was stimulated by IGF-I, but not insulin, at 1 nM. Accumulation of glucose in PA was increased by insulin at 10 nM and by IGF-I at 1 nM and 10 nM. DNA synthesis was increased by insulin and IGF-I at 10 nM. In conclusion, both IR and IGF-IR are present in human preadipocytes and adipocytes. Differentiation is characterized by an increased IR/IGF-IR ratio.
Ämnesord och genrebeteckningar
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Insulin receptor isoforms
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Cell metabolism
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Growth factors
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MEDICINE
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MEDICIN
Biuppslag (personer, institutioner, konferenser, titlar ...)
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Arnqvist, HansÖstergötlands Läns Landsting,Linköpings universitet,Cellbiologi,Hälsouniversitetet,Endokrin- och magtarmmedicinska kliniken US(Swepub:liu)hanar64
(författare)
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Linköpings universitetCellbiologi
(creator_code:org_t)
Sammanhörande titlar
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Ingår i:Growth Hormone & IGF Research: Elsevier BV19:2, s. 101-1111096-63741532-2238
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