L773:1532 2238
Sökning: L773:1532 2238 > Changes in insulin ...
LIBRIS Formathandbok (Information om MARC21)
| Fältnamn | Indikatorer | Metadata |
|---|---|---|
| 000 | 03479naa a2200361 4500 | |
| 001 | oai:DiVA.org:liu-17895 | |
| 003 | SwePub | |
| 008 | 090424s2009 | |||||||||||000 ||eng| | |
| 024 | 7 | a https://urn.kb.se/resolve?urn=urn:nbn:se:liu:diva-178952 URI |
| 024 | 7 | a https://doi.org/10.1016/j.ghir.2008.06.0042 DOI |
| 040 | a (SwePub)liu | |
| 041 | a engb eng | |
| 042 | 9 SwePub | |
| 072 | 7 | a ref2 swepub-contenttype |
| 072 | 7 | a art2 swepub-publicationtype |
| 100 | 1 | a Bäck, Karolinau Linköpings universitet,Cellbiologi,Hälsouniversitetet4 aut0 (Swepub:liu)karol97 |
| 245 | 1 0 | a Changes in insulin and IGF-I receptor expression during differentiation of human preadipocytes |
| 264 | 1 | b Elsevier BV,c 2009 |
| 338 | a electronic2 rdacarrier | |
| 500 | a Original Publication: Karolina Bäck and Hans Arnqvist, Changes in insulin and IGF-I receptor expression during differentiation of human preadipocytes, 2009, GROWTH HORMONE and IGF RESEARCH, (19), 2, 101-111. http://dx.doi.org/10.1016/j.ghir.2008.06.004 Copyright: Elsevier Science B.V., Amsterdam http://www.elsevier.com/ | |
| 520 | a Mature adipocytes originate from fibroblast-like precursor cells, preadipocytes, which differentiate to obtain the characteristics of adipocytes. Our aim was to investigate how differentiation of human preadipocytes affects the distribution of insulin receptors (IR) and IGF-I receptors (IGF-IR) and other cell characteristics. Preadipocytes were differentiated using indomethacine, dexamethasone, isobutyl-methylxantine (IBMX) and high concentration of insulin. Gene expression was quantified by real-time RT-PCT in preadipocytes (PA), differentiated preadipocytes (dPA) and mature adipocytes (mAD). The amount of expressed receptor protein was analyzed using receptor specific ELISAs and Western blot. We also studied DNA synthesis with radiolabeled thymidine incorporation and glucose accumulation with radiolabeled glucose. Differentiation of PA increased gene expression of IR but not IGF-IR, GLUT4, growth hormone receptor (GHR) and adiponectin appeared or increased. In PA and dPA only IR-A was expressed whereas also IR-B was detected in mAD. By Western blot and ELISA, IR and IGF-IR was phosphorylated by their own ligant at 1 nM and in dPA the acitivation of both receptors was stimulated by IGF-I, but not insulin, at 1 nM. Accumulation of glucose in PA was increased by insulin at 10 nM and by IGF-I at 1 nM and 10 nM. DNA synthesis was increased by insulin and IGF-I at 10 nM. In conclusion, both IR and IGF-IR are present in human preadipocytes and adipocytes. Differentiation is characterized by an increased IR/IGF-IR ratio. | |
| 653 | a Insulin receptor isoforms | |
| 653 | a Cell metabolism | |
| 653 | a Growth factors | |
| 653 | a MEDICINE | |
| 653 | a MEDICIN | |
| 700 | 1 | a Arnqvist, Hansu Östergötlands Läns Landsting,Linköpings universitet,Cellbiologi,Hälsouniversitetet,Endokrin- och magtarmmedicinska kliniken US4 aut0 (Swepub:liu)hanar64 |
| 710 | 2 | a Linköpings universitetb Cellbiologi4 org |
| 773 | 0 | t Growth Hormone & IGF Researchd : Elsevier BVg 19:2, s. 101-111q 19:2<101-111x 1096-6374x 1532-2238 |
| 856 | 4 | u https://liu.diva-portal.org/smash/get/diva2:212984/FULLTEXT01.pdfx primaryx Raw objecty fulltext:postprint |
| 856 | 4 | u http://liu.diva-portal.org/smash/get/diva2:212984/FULLTEXT01 |
| 856 | 4 8 | u https://urn.kb.se/resolve?urn=urn:nbn:se:liu:diva-17895 |
| 856 | 4 8 | u https://doi.org/10.1016/j.ghir.2008.06.004 |
Hitta via bibliotek
- Growth Hormone & IGF Research (Sök värdpublikationen i LIBRIS)
Till lärosätets databas
Hitta mer i SwePub
- Av författaren/redakt...
- Bäck, Karolina
- Arnqvist, Hans
- Artiklar i publikationen
- Growth Hormone & ...
- Av lärosätet
- Linköpings universitet
Sök utanför SwePub
- Sök vidare i:
- Google Book Search
- Google Scholar
Kungliga biblioteket hanterar dina personuppgifter i enlighet med EU:s dataskyddsförordning (2018), GDPR. Läs mer om hur det funkar här.
Så här hanterar KB dina uppgifter vid användning av denna tjänst.
- Copyright © LIBRIS - Nationella bibliotekssystem
- LIBRIS.kb.se
