L773:1879 0089
Sökning: L773:1879 0089 > Two granzyme A/K ho...
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| Fältnamn | Indikatorer | Metadata |
|---|---|---|
| 000 | 05524naa a2200529 4500 | |
| 001 | oai:DiVA.org:uu-514069 | |
| 003 | SwePub | |
| 008 | 231016s2023 | |||||||||||000 ||eng| | |
| 009 | oai:slubar.slu.se:126351 | |
| 024 | 7 | a https://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-5140692 URI |
| 024 | 7 | a https://doi.org/10.1016/j.dci.2023.1049202 DOI |
| 024 | 7 | a https://res.slu.se/id/publ/1263512 URI |
| 040 | a (SwePub)uud (SwePub)slu | |
| 041 | a engb eng | |
| 042 | 9 SwePub | |
| 072 | 7 | a ref2 swepub-contenttype |
| 072 | 7 | a art2 swepub-publicationtype |
| 100 | 1 | a Aybay, Erdemu Uppsala universitet,Institutionen för cell- och molekylärbiologi4 aut |
| 245 | 1 0 | a Two granzyme A/K homologs in Zebra mbuna have different specificities, one classical tryptase and one with chymase activity |
| 264 | 1 | b Elsevier,c 2023 |
| 338 | a electronic2 rdacarrier | |
| 520 | a Granzymes A and K are two highly homologous serine proteases expressed by mammalian cytotoxic T cells (CTLs) and natural killer (NK) cells. The locus encoding these two proteases is the first of the hematopoietic serine protease loci to appear during vertebrate evolution. This locus is found in all jawed vertebrates including the cartilaginous fishes. Granzyme A is the most abundant of the different granzymes expressed by CTLs and NK cells and its potential function has been studied extensively for many years. However, no clear conclusions concerning its primary role in the immune defense has been obtained. In all mammals, there are only one copy each of granzyme A and K, whereas additional copies are found in both cartilaginous and ray finned fishes. In cichlids two of these copies seem to encode new members of the granzyme A/K family. These two new members appear to have changed primary specificity and to be pure chymases based on the amino acids in their active site substrate binding pockets. Interestingly, one of these gene copies is located in the middle of the granzyme A/K locus, while the other copy is present in another locus, the met-ase locus. We here present a detailed characterization of the extended cleavage specificity of one of these non-classical granzymes, a Zebra mbuna granzyme positioned in the granzyme A/K locus. This enzyme, named granzyme A2, showed a high preference for tyrosine in the P1 position of substrates, thereby being a strict chymase. We have also characterized one of the classical granzyme A/Ks of the Zebra mbuna, granzyme A1, which is a tryptase with preference for arginine in the P1 position of substrates. Based on their extended specificities, the two granzymes showed major similarities, but also some differences in preferred amino acids in positions surrounding the cleavable amino acid. Fish lack one of the hematopoietic serine protease loci of mammals, the chymase locus, where one of the major mast cell enzymes is located. An interesting question is now if cichlids have by compensatory mechanisms generated a mast cell chymase from another locus, and if similar chymotryptic enzymes have appeared also in other fish species. | |
| 650 | 7 | a NATURVETENSKAPx Biologix Immunologi0 (SwePub)106052 hsv//swe |
| 650 | 7 | a NATURAL SCIENCESx Biological Sciencesx Immunology0 (SwePub)106052 hsv//eng |
| 650 | 7 | a NATURVETENSKAPx Biologix Biokemi och molekylärbiologi0 (SwePub)106022 hsv//swe |
| 650 | 7 | a NATURAL SCIENCESx Biological Sciencesx Biochemistry and Molecular Biology0 (SwePub)106022 hsv//eng |
| 650 | 7 | a LANTBRUKSVETENSKAPERx Lantbruksvetenskap, skogsbruk och fiskex Fisk- och akvakulturforskning0 (SwePub)401072 hsv//swe |
| 650 | 7 | a AGRICULTURAL SCIENCESx Agriculture, Forestry and Fisheriesx Fish and Aquacultural Science0 (SwePub)401072 hsv//eng |
| 653 | a Cytotoxic T cells | |
| 653 | a NK cell | |
| 653 | a Granzyme A | |
| 653 | a Apoptosis | |
| 653 | a Caspase | |
| 653 | a Chymase | |
| 700 | 1 | a Elkhalifa, Mamounu Uppsala universitet,Institutionen för cell- och molekylärbiologi4 aut |
| 700 | 1 | a Akula, Srinivasu Swedish University of Agricultural Sciences,Sveriges lantbruksuniversitet,Uppsala universitet,Mikrobiologi och immunologi,Swedish Univ Agr Sci, Dept Anat Physiol & Biochem, Box 7011, SE-75007 Uppsala, Sweden.,Institutionen för anatomi, fysiologi och biokemi (AFB),Department of Anatomy, Physiology and Biochemistry (AFB),Uppsala University4 aut0 (Swepub:uu)akusr139 |
| 700 | 1 | a Wernersson, Sarau Swedish University of Agricultural Sciences,Sveriges lantbruksuniversitet,Institutionen för anatomi, fysiologi och biokemi (AFB),Department of Anatomy, Physiology and Biochemistry (AFB),Swedish Univ Agr Sci, Dept Anat Physiol & Biochem, Box 7011, SE-75007 Uppsala, Sweden.4 aut0 (Swepub:slu)48577 |
| 700 | 1 | a Hellman, Larsu Uppsala universitet,Mikrobiologi och immunologi4 aut0 (Swepub:uu)larshell |
| 710 | 2 | a Uppsala universitetb Institutionen för cell- och molekylärbiologi4 org |
| 710 | 2 | a Sveriges lantbruksuniversitet |
| 773 | 0 | t Developmental and Comparative Immunologyd : Elsevierg 148q 148x 0145-305Xx 1879-0089 |
| 856 | 4 | u https://doi.org/10.1016/j.dci.2023.104920y Fulltext |
| 856 | 4 | u https://uu.diva-portal.org/smash/get/diva2:1805169/FULLTEXT01.pdfx primaryx Raw objecty fulltext:print |
| 856 | 4 | u https://pub.epsilon.slu.se/id/eprint/31782/contentsx primaryx Raw objectx freey FULLTEXT |
| 856 | 4 8 | u https://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-514069 |
| 856 | 4 8 | u https://doi.org/10.1016/j.dci.2023.104920 |
| 856 | 4 8 | u https://res.slu.se/id/publ/126351 |
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