WFRF:(Czolkos Ilja)
Sökning: WFRF:(Czolkos Ilja) > Direct immobilizati...
Direct immobilization of cholesteryl-TEG-modified oligonucleotides onto hydrophobic SU-8 surfaces
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- Erkan, Yavuz, 1982 (författare)
- Chalmers tekniska högskola,Chalmers University of Technology
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- Czolkos, Ilja, 1980 (författare)
- Chalmers tekniska högskola,Chalmers University of Technology
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- Jesorka, Aldo, 1967 (författare)
- Chalmers tekniska högskola,Chalmers University of Technology
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- (creator_code:org_t)
- 2007-04-14
- 2007
- Engelska.
- Ingår i: Langmuir. - : American Chemical Society (ACS). - 1520-5827 .- 0743-7463. ; 23:10, s. 5259-5263
- Tidskriftsartikel (refereegranskat)
Abstract
Ämnesord
Stäng
- We introduce a rapid, simple one-step procedure for the high-yield immobilization of cholesteryl- tetraethyleneglycol-modified oligonucleotides ( chol- DNA) at hydrophobic sites made of SU-8 photoresist. Topographic structures of SU-8 were microfabricated on microscope glass coverslips sputtered with a Ti/Au layer. Upon application, chol-DNA adsorbed to the SU-8 structures from solution, leaving the surrounding gold surface free of chol- DNA. chol-DNA immobilization is complete within 15 min and yields a surface coverage in the range of 20- 95 pmol/ cm(2), which corresponds to a film density of 10(12)- 10(13) molecules/cm(2). chol-DNA immobilization is stable and can be sustained despite rinsing, drying, dry storage for several hours, and rehydration of chips. Furthermore, complementary DNA in solution hybridizes efficiently to immobilized chol-DNA. We introduce a rapid, simple one-step procedure for the high-yield immobilization of cholesteryl-tetraethyleneglycol-modified oligonucleotides (chol-DNA) at hydrophobic sites made of SU-8 photoresist. Topographic structures of SU-8 were microfabricated on microscope glass coverslips sputtered with a Ti/Au layer. Upon application, chol-DNA adsorbed to the SU-8 structures from solution, leaving the surrounding gold surface free of chol-DNA. chol-DNA immobilization is complete within 15 min and yields a surface coverage in the range of 20-95 pmol/cm(2), which corresponds to a film density of 10(12)-10(13) molecules/cm(2). chol-DNA immobilization is stable and can be sustained despite rinsing, drying, dry storage for several hours, and rehydration of chips. Furthermore, complementary DNA in solution hybridizes efficiently to immobilized chol-DNA.
Ämnesord
- NATURVETENSKAP -- Kemi (hsv//swe)
- NATURAL SCIENCES -- Chemical Sciences (hsv//eng)
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- Erkan, Yavuz, 19 ...
- Czolkos, Ilja, 1 ...
- Jesorka, Aldo, 1 ...
- Wilhelmsson, Mar ...
- Orwar, Owe, 1964
- Om ämnet
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- NATURVETENSKAP
- NATURVETENSKAP
- och Kemi
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- Langmuir
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- Chalmers tekniska högskola
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