Two-photon excitation and time-resolved fluorescence: I. The proper response function for analysing single-photon counting experiments

Habenicht, Anja (författare)

Hjelm, Johan (författare)

Mukhtar, Emad (författare)

(creator_code:org_t)

Relaterad länk:: https://urn.kb.se/re...; visa fler...; https://doi.org/10.1...; visa färre...

Abstract Ämnesord

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An accurate instrumental response function is needed to deconvolute fluorescence data obtained by time-correlated single-photon counting (TCSPC) upon multi-photon excitation. Hitherto the response function was obtained by measuring Rayleigh scattering (RS) from colloidal solutions, as is also used in one-photon excited fluorescence. We show that hyper Rayleigh scattering (HRS) provides a better choice for deconvolution of fluorescence decays, as obtained by TCSPC and two-photon excitation (TPE). The one- and two-photon response functions were monitored as RS and HRS from colloidal gold particles at 800 and 400 nm, respectively.

Av författaren/redakt...: Habenicht, Anja; Hjelm, Johan; Mukhtar, Emad; Bergström, Fredr ...; Johansson, Lenna ...

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