WFRF:(Howard Rebecca J.)
Search: WFRF:(Howard Rebecca J.) > (2010-2014) > Genotyping for CYP2...
- 9 of 14
- Previous record
- Next record
- To hitlist
Genotyping for CYP2C9 and VKORC1 alleles by a novel point of care assay with HyBeacon® probes
- Howard, Rebecca (author)
- Leathart, Julian B S (author)
- French, David J (author)
- show more...
- Krishan, Elaina (author)
- van Schie, Rianne (author)
- Verhoef, Talitha (author)
- Maitland-van der Zee, Anke-Hilse (author)
- Daly, Ann K (author)
- Barallon, Rita (author)
- show less...
- (creator_code:org_t)
- Elsevier BV, 2011
- 2011
- English.
- In: Clinica Chimica Acta. - : Elsevier BV. - 0009-8981 .- 1873-3492. ; 412:23-24, s. 2063-2069
- Journal article (peer-reviewed)
Abstract
Subject headings
Close
- BACKGROUND: Coumarin anticoagulants such as warfarin are used to treat and prevent thromboembolic events in patients. The required dosage is difficult to predict and the risk of over or under anticoagulation are dependent on several environmental and clinical factors, such as concurrent medication, diet, age and genotype for polymorphisms in two genes CYP2C9 and VKORC1.METHODS: A novel fluorescent PCR genotyping assay using HyBeacon® probes, was developed to enable clinical staff to genotype the CYP2C9*2 and CYP2C9*3 alleles and the VKORC1 G-1639A polymorphism directly from unextracted blood samples. A prototype PCR instrument, Genie 1, suitable for point of care use was developed to carry out the assays. The panel of tests was validated by analysing blood samples from 156 individuals and comparing genotypes with data obtained using DNA samples from the same individuals. The accuracy of genotypes obtained with the Genie 1 was compared against results from well validated real time PCR and PCR-restriction fragment length polymorphism analysis.RESULTS: Identical results were obtained for the newly developed HyBeacon® method and the validation method in all cases except for one where no result was obtained for the VKORC1 polymorphism on the Genie instrument. The samples used for validation represented all six possible *2 and *3 allele-related CYP2C9 genotypes and all three VKORC1 G-1639A genotypes.CONCLUSIONS: We observed excellent accuracy for the newly developed method which can determine genotype in less than 2 h.
Subject headings
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Farmakologi och toxikologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Pharmacology and Toxicology (hsv//eng)
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinsk bioteknologi -- Biomedicinsk laboratorievetenskap/teknologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Medical Biotechnology -- Biomedical Laboratory Science/Technology (hsv//eng)
Keyword
- Warfarin
- Phenprocoumon
- Acenocoumarol
- CYP2C9
- VKORC1
- Point of care genotyping
Publication and Content Type
- ref (subject category)
- art (subject category)
Find in a library
- Clinica Chimica Acta (Search for host publication in LIBRIS)
To the university's database
- 9 of 14
- Previous record
- Next record
- To hitlist
Find more in SwePub
- By the author/editor
- Howard, Rebecca
- Leathart, Julian ...
- French, David J
- Krishan, Elaina
- Kohnke, Hugo
- Wadelius, Mia
- show more...
- van Schie, Riann ...
- Verhoef, Talitha
- Maitland-van der ...
- Daly, Ann K
- Barallon, Rita
- show less...
- About the subject
-
- MEDICAL AND HEALTH SCIENCES
- MEDICAL AND HEAL ...
- and Basic Medicine
- and Pharmacology and ...
-
- MEDICAL AND HEALTH SCIENCES
- MEDICAL AND HEAL ...
- and Medical Biotechn ...
- and Biomedical Labor ...
- Articles in the publication
- Clinica Chimica ...
- By the university
- Uppsala University
Search outside SwePub
- Extend your search to:
- Google Book Search
- Google Scholar
Kungliga biblioteket hanterar dina personuppgifter i enlighet med EU:s dataskyddsförordning (2018), GDPR. Läs mer om hur det funkar här.
Så här hanterar KB dina uppgifter vid användning av denna tjänst.
- Copyright © LIBRIS - National Library Systems
- LIBRIS.kb.se
