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Invasiveness and me...
Invasiveness and metastasis of retinoblastoma in an orthotopic zebrafish tumor model
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- Chen, Xiaoyun (author)
- Sun Yat Sen University, Peoples R China; Karolinska Institute, Sweden
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- Wang, Jian (author)
- Karolinska Institute, Sweden; Shandong University, Peoples R China
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- Cao, Ziquan (author)
- Linköpings universitet,Avdelningen för kardiovaskulär medicin,Medicinska fakulteten,Karolinska Institute, Sweden
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- Hosaka, Kayoko (author)
- Karolinska Institutet,Karolinska Institute, Sweden
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- Jensen, Lasse (author)
- Linköpings universitet,Avdelningen för kardiovaskulär medicin,Medicinska fakulteten,Karolinska Institute, Sweden
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- Yang, Huasheng (author)
- Karolinska Institutet,Sun Yat Sen University, Peoples R China
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- Sun, Yuping (author)
- Shandong University, Peoples R China
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- Zhuang, Rujie (author)
- Chinese Medical University, Peoples R China
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- Liu, Yizhi (author)
- Sun Yat Sen University, Peoples R China
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- Cao, Yihai (author)
- Linköpings universitet,Avdelningen för kardiovaskulär medicin,Medicinska fakulteten,Karolinska Institute, Sweden; University of Leicester, England; Glenfield Hospital, England
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(creator_code:org_t)
- 2015-07-14
- 2015
- English.
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In: Scientific Reports. - : Nature Publishing Group: Open Access Journals - Option C / Nature Publishing Group. - 2045-2322. ; 5:10351
- Related links:
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https://www.nature.c...
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https://urn.kb.se/re...
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Abstract
Subject headings
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- Retinoblastoma is a highly invasive malignant tumor that often invades the brain and metastasizes to distal organs through the blood stream. Invasiveness and metastasis of retinoblastoma can occur at the early stage of tumor development. However, an optimal preclinical model to study retinoblastoma invasiveness and metastasis in relation to drug treatment has not been developed. Here, we developed an orthotopic zebrafish model in which retinoblastoma invasion and metastasis can be monitored at a single cell level. We took the advantages of immune privilege and transparent nature of developing zebrafish embryos. Intravitreal implantation of color-coded retinoblastoma cells allowed us to kinetically monitor tumor cell invasion and metastasis. Further, interactions between retinoblastoma cells and surrounding microvasculatures were studied using a transgenic zebrafish that exhibited green fluorescent signals in blood vessels. We discovered that tumor cells invaded neighboring tissues and blood stream when primary tumors were at the microscopic sizes. These findings demonstrate that retinoblastoma metastasis occurs at the early stage and antiangiogenic drugs such as Vegf morpholino and sunitinib could potentially interfere with tumor invasiveness and metastasis. Thus, this orthotopic retinoblastoma model offers a new and unique opportunity to study the early events of tumor invasion, metastasis and drug responses.
Subject headings
- MEDICIN OCH HÄLSOVETENSKAP -- Klinisk medicin (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Clinical Medicine (hsv//eng)
Publication and Content Type
- ref (subject category)
- art (subject category)
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- By the author/editor
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Chen, Xiaoyun
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Wang, Jian
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Cao, Ziquan
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Hosaka, Kayoko
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Jensen, Lasse
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Yang, Huasheng
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Sun, Yuping
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Zhuang, Rujie
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Liu, Yizhi
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Cao, Yihai
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- About the subject
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- MEDICAL AND HEALTH SCIENCES
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MEDICAL AND HEAL ...
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and Clinical Medicin ...
- Articles in the publication
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Scientific Repor ...
- By the university
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Linköping University
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Karolinska Institutet