WFRF:(Kieseier BC)
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| Fältnamn | Indikatorer | Metadata |
|---|---|---|
| 000 | 02685naa a2200325 4500 | |
| 001 | oai:prod.swepub.kib.ki.se:128082502 | |
| 003 | SwePub | |
| 008 | 240701s2013 | |||||||||||000 ||eng| | |
| 024 | 7 | a http://kipublications.ki.se/Default.aspx?queryparsed=id:1280825022 URI |
| 024 | 7 | a https://doi.org/10.1177/13524585134754892 DOI |
| 040 | a (SwePub)ki | |
| 041 | a engb eng | |
| 042 | 9 SwePub | |
| 072 | 7 | a ref2 swepub-contenttype |
| 072 | 7 | a art2 swepub-publicationtype |
| 100 | 1 | a Warnke, C4 aut |
| 245 | 1 0 | a An assay to quantify species-specific anti-JC virus antibody levels in MS patients |
| 264 | c 2013-02-06 | |
| 264 | 1 | b SAGE Publications,c 2013 |
| 520 | a The StratifyJCV® test is a qualitative assay to classify MS patients as anti-JC virus (JCV) antibody positive or negative. Quantification of anti-JCV antibody levels in serum and cerebrospinal fluid (CSF) of multiple sclerosis (MS) patients might add to the progressive multifocal leukoencephalopathy (PML) risk assessment.Objective:The objective of this study is to test sera of patients in a quantitative anti-JCV antibody assay, and to compare the results with preexisting data from the StratifyJCV® test.Methods:Sera of a total of 175 MS patients and matched non-MS-controls were tested for anti-JCV antibodies using glutathione S-transferase-tagged-VP1 as antigen. Antibody reactivity was quantified in arbitrary units using human immunoglobulin as standard.Results:The comparison of our assay with StratifyJCV® showed good inter-assay agreement (kappa 0.6), and strong correlation for antibody reactivity ( r2= 0.94). Discordant samples had low-reactive positivity, and a higher proportion (13% vs. 4%) tested positive in the StratifyJCV® test only.Conclusions:The method presented is a tool for the reliable quantification of anti-JCV antibodies, which demonstrates good agreement with results from StratifyJCV®. In contrast to StratifyJCV®, we pre-adsorbed all of the sera with BK virus (BKV) VP1 protein to reduce cross-reactivity. This step may account for a higher species-specificity of our assay. As such, our assay might be a promising additional tool for PML risk assessment. | |
| 700 | 1 | a Pawlita, M4 aut |
| 700 | 1 | a Dehmel, T4 aut |
| 700 | 1 | a Posevitz-Fejfar, A4 aut |
| 700 | 1 | a Hartung, HP4 aut |
| 700 | 1 | a Wiendl, H4 aut |
| 700 | 1 | a Kieseier, BC4 aut |
| 700 | 1 | a Adams, O4 aut |
| 773 | 0 | t Multiple sclerosis (Houndmills, Basingstoke, England)d : SAGE Publicationsg 19:9, s. 1137-1144q 19:9<1137-1144x 1477-0970x 1352-4585 |
| 856 | 4 8 | u http://kipublications.ki.se/Default.aspx?queryparsed=id:128082502 |
| 856 | 4 8 | u https://doi.org/10.1177/1352458513475489 |
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- Warnke, C
- Pawlita, M
- Dehmel, T
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- Hartung, HP
- Wiendl, H
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- Kieseier, BC
- Adams, O
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- Karolinska Institutet
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