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Ultrafiltration with size-exclusion liquid chromatography for high yield isolation of extracellular vesicles preserving intact biophysical and functional properties
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- Nordin, Joel Z. (author)
- Karolinska Institutet
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- Lee, Yi (author)
- Karolinska Institutet
- Vader, Pieter (author)
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- Mäger, Imre (author)
- Heusermann, Wolf (author)
- Seow, Yiqi (author)
- Bultema, Jarred J. (author)
- Davies, Tim (author)
- Fairchild, Paul J. (author)
- Meisner-Kober, Nicole C. (author)
- Wood, Matthew J. A. (author)
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- (creator_code:org_t)
- Elsevier BV, 2015
- 2015
- English.
- In: Nanomedicine. - : Elsevier BV. - 1549-9634 .- 1549-9642. ; 11:4, s. 879-883
- Journal article (peer-reviewed)
Abstract
Subject headings
Close
- Extracellular vesicles (EVs) are natural nanoparticles that mediate intercellular transfer of RNA and proteins and are of great medical interest; serving as novel biomarkers and potential therapeutic agents. However, there is little consensus on the most appropriate method to isolate high-yield and high-purity EVs from various biological fluids. Here, we describe a systematic comparison between two protocols for EV purification: ultrafiltration with subsequent liquid chromatography (UF-LC) and differential ultracentrifugation (UC). A significantly higher EV yield resulted from UF-LC as compared to UC, without affecting vesicle protein composition. Importantly, we provide novel evidence that, in contrast to UC-purified EVs, the biophysical properties of UF-LC-purified EVs are preserved, leading toadifferent in vivo biodistribution, with less accumulation in lungs. Finally, we show that UF-LC is scalable and adaptable for EV isolation from complex media types such as stem cell media, which is of huge significance for future clinical applications involving EVs.
Subject headings
- NATURVETENSKAP -- Kemi (hsv//swe)
- NATURAL SCIENCES -- Chemical Sciences (hsv//eng)
- NATURVETENSKAP -- Biologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences (hsv//eng)
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinsk bioteknologi -- Medicinsk bioteknologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Medical Biotechnology -- Medical Biotechnology (hsv//eng)
Keyword
- Biophysical properties
- extracellular vesicles
- size-exclusion liquid chromatography
- ultracentrifugation
- ultrafiltration
Publication and Content Type
- ref (subject category)
- art (subject category)
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- Nanomedicine
- By the university
- Stockholm University
- Umeå University
- Karolinska Institutet
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