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Protein profiles of...
Protein profiles of CCL5, HPGDS, and NPSR1 in plasma reveal association with childhood asthma
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- Hamsten, C. (author)
- Karolinska Institutet,Immunology and Allergy Unit; Department of Medicine Solna; Karolinska Institutet; and Karolinska University Hospital;Center for Inflammatory Diseases; Karolinska Institutet; Stockholm Sweden
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- Häggmark, Anna, 1985- (author)
- KTH,Science for Life Laboratory, SciLifeLab,Affinitets-proteomik,KTH Royal Inst Technol, Sch Biotechnol, SciLifeLab, Affin Prote, Stockholm, Sweden.,Affinity Proteomics; SciLifeLab; School of Biotechnology; KTH-Royal Institute of Technology; Stockholm Sweden
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- Grundstrom, J. (author)
- Karolinska Institutet,Immunology and Allergy Unit; Department of Medicine Solna; Karolinska Institutet; and Karolinska University Hospital
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- Mikus, Maria (author)
- Karolinska Institutet,KTH,Proteomik och nanobioteknologi,KTH Royal Inst Technol, Sch Biotechnol, SciLifeLab, Affin Prote, Stockholm, Sweden.,Karolinska Inst, Inst Environm Med, Stockholm, Sweden.;Soder Sjukhuset, Sachs Children & Youth Hosp, Stockholm, Sweden.,Karolinska Inst, Immunol & Allergy Unit, Dept Med Solna, Stockholm, Sweden.;Karolinska Univ Hosp, Stockholm, Sweden.,Affinity Proteomics; SciLifeLab; School of Biotechnology; KTH-Royal Institute of Technology; Stockholm Sweden,Institute of Environmental Medicine; Karolinska Institutet; Stockholm Sweden;Sachs’ Children and Youth Hospital; Södersjukhuset; Stockholm Sweden,Immunology and Allergy Unit; Department of Medicine Solna; Karolinska Institutet; and Karolinska University Hospital
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- Lindskog, Cecilia (author)
- Uppsala universitet,Institutionen för immunologi, genetik och patologi,Science for Life Laboratory, SciLifeLab,Cancerprecisionsmedicin,SciLifeLab; Department of Immunology; Genetics and Pathology; Uppsala University; Uppsala Sweden
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- Konradsen, J. R. (author)
- Karolinska Institutet,Astrid Lindgren Children's Hospital; Karolinska University Hospital; Stockholm Sweden;Department of Women's and Children's Health; Karolinska Institutet; Stockholm Sweden
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- Eklund, A. (author)
- Karolinska Institutet,Respiratory Medicine Unit; Department of Medicine Solna and CMM; Karolinska Institutet and Karolinska University Hospital; Stockholm Sweden
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- Pershagen, G. (author)
- Karolinska Institutet,Institute of Environmental Medicine; Karolinska Institutet; Stockholm Sweden
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Wickman, M. (author)
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- Grunewald, J. (author)
- Karolinska Institutet,Respiratory Medicine Unit; Department of Medicine Solna and CMM; Karolinska Institutet and Karolinska University Hospital; Stockholm Sweden
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- Melen, E. (author)
- Karolinska Institutet,Institute of Environmental Medicine; Karolinska Institutet; Stockholm Sweden;Sachs’ Children and Youth Hospital; Södersjukhuset; Stockholm Sweden
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- Hedlin, G. (author)
- Karolinska Institutet,Astrid Lindgren Children's Hospital; Karolinska University Hospital; Stockholm Sweden;Department of Women's and Children's Health; Karolinska Institutet; Stockholm Sweden
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- Nilsson, Peter (author)
- KTH,Proteomik och nanobioteknologi,KTH Royal Inst Technol, Sch Biotechnol, SciLifeLab, Affin Prote, Stockholm, Sweden.,Affinity Proteomics; SciLifeLab; School of Biotechnology; KTH-Royal Institute of Technology; Stockholm Sweden
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van Hage, M. (author)
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(creator_code:org_t)
- 2016-05-25
- 2016
- English.
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In: Allergy. European Journal of Allergy and Clinical Immunology. - : Blackwell Publishing. - 0105-4538 .- 1398-9995. ; 71:9, s. 1357-1361
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https://doi.org/10.1...
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Abstract
Subject headings
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- Asthma is a common chronic childhood disease with many different phenotypes that need to be identified. We analyzed a broad range of plasma proteins in children with well-characterized asthma phenotypes to identify potential markers of childhood asthma. Using an affinity proteomics approach, plasma levels of 362 proteins covered by antibodies from the Human Protein Atlas were investigated in a total of 154 children with persistent or intermittent asthma and controls. After screening, chemokine ligand 5 (CCL5) hematopoietic prostaglandin D synthase (HPGDS) and neuropeptide S receptor 1 (NPSR1) were selected for further investigation. Significantly lower levels of both CCL5 and HPGDS were found in children with persistent asthma, while NPSR1 was found at higher levels in children with mild intermittent asthma compared to healthy controls. In addition, the protein levels were investigated in another respiratory disease, sarcoidosis, showing significantly higher NPSR1 levels in sera from sarcoidosis patients compared to healthy controls. Immunohistochemical staining of healthy tissues revealed high cytoplasmic expression of HPGDS in mast cells, present in stroma of both airway epithelia, lung as well as in other organs. High expression of NPSR1 was observed in neuroendocrine tissues, while no expression was observed in airway epithelia or lung. In conclusion, we have utilized a broad-scaled affinity proteomics approach to identify three proteins with altered plasma levels in asthmatic children, representing one of the first evaluations of HPGDS and NPSR1 protein levels in plasma.
Subject headings
- NATURVETENSKAP -- Biologi -- Immunologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Immunology (hsv//eng)
- MEDICIN OCH HÄLSOVETENSKAP -- Klinisk medicin -- Lungmedicin och allergi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Clinical Medicine -- Respiratory Medicine and Allergy (hsv//eng)
Keyword
- asthma
- biomarker
- childhood
- plasma
- protein
- Biology with specialization in Molecular Biology
Publication and Content Type
- ref (subject category)
- art (subject category)
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To the university's database
- By the author/editor
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Hamsten, C.
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Häggmark, Anna, ...
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Grundstrom, J.
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Mikus, Maria
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Lindskog, Cecili ...
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Konradsen, J. R.
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show more...
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Eklund, A.
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Pershagen, G.
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Wickman, M.
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Grunewald, J.
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Melen, E.
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Hedlin, G.
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Nilsson, Peter
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van Hage, M.
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- About the subject
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- NATURAL SCIENCES
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NATURAL SCIENCES
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and Biological Scien ...
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and Immunology
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- MEDICAL AND HEALTH SCIENCES
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MEDICAL AND HEAL ...
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and Clinical Medicin ...
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and Respiratory Medi ...
- Articles in the publication
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Allergy. Europea ...
- By the university
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Royal Institute of Technology
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Uppsala University
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Karolinska Institutet