Demonstration of mitochondrial oestrogen receptor beta and oestrogen-induced attenuation of cytochrome c oxidase subunit I expression in human periodontal ligament cells.

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Jönsson, DanielMalmö högskola,Lund University,Lunds universitet,Kärlfysiologi,Forskargrupper vid Lunds universitet,Vascular Physiology,Lund University Research Groups,Odontologiska fakulteten (OD) (author)

Demonstration of mitochondrial oestrogen receptor beta and oestrogen-induced attenuation of cytochrome c oxidase subunit I expression in human periodontal ligament cells.

Article/chapterEnglish2007

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Elsevier BV,2007

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LIBRIS-ID:oai:lup.lub.lu.se:3dd7cae9-8b7d-4c16-9917-266532ec4fa1
https://lup.lub.lu.se/record/164901URI
https://doi.org/10.1016/j.archoralbio.2006.12.009DOI
https://urn.kb.se/resolve?urn=urn:nbn:se:mau:diva-15609URI

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Language:English
Summary in:English

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Subject category:art swepub-publicationtype
Subject category:ref swepub-contenttype

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OBJECTIVE: Periodontal ligament (PDL) cells express oestrogen receptor beta (ERbeta) protein, but cellular functions regulated by ERbeta in these cells have not been identified. In this study we determine if ERbeta is localised to mitochondria and if oestrogen regulates mitochondrial function in human PDL cells obtained from teeth extracted for orthodontic reasons. DESIGN: Subcellular distribution of ERbeta was determined by confocal microscopy of cells co-stained with ERbeta antibody and the mitochondrion-selective probe MitoTracker and by immunogold electron microscopy. Expression of the mitochondrial enzyme cytochrome c oxidase subunit I, involved in oxidative phosphorylation, was determined by Western blotting in cells treated with or without physiological concentrations of the endogenous oestrogen 17beta-oestradiol. RESULTS: ERbeta immunoreactivity was observed both in the nuclei and the cytoplasm. MitoTracker-labelling was observed in the cytoplasm, especially in the perinuclear region, but not in the nuclei. Co-localisation of ERbeta and MitoTracker was observed in cells derived from both male and female subjects. Mitochondrial localisation of ERbeta was confirmed by immunogold electron microscopy. Cells treated with or without 17beta-oestradiol (100 nM) displayed an identical pattern of staining for mitochondria. Treatment with 100 nM 17beta-oestradiol attenuated cytochrome c oxidase subunit I expression by about 30%, while combined treatment with 17beta-oestradiol and the ER blocker ICI 182780 (10 microM) had no effect. CONCLUSION: This study demonstrates mitochondrial localisation of ERbeta and oestrogen-induced decrease in the expression of cytochrome c oxidase subunit I in human PDL cells, suggesting that oestrogen probably via ERbeta influences mitochondrial function and PDL cell energy

Subject headings and genre

MEDICIN OCH HÄLSOVETENSKAP Medicinska och farmaceutiska grundvetenskaper Fysiologi hsv//swe
MEDICAL AND HEALTH SCIENCES Basic Medicine Physiology hsv//eng
MEDICIN OCH HÄLSOVETENSKAP Klinisk medicin Hematologi hsv//swe
MEDICAL AND HEALTH SCIENCES Clinical Medicine Hematology hsv//eng
Oxidative phosphorylation
Mitochondria
Oestrogen
Immunocytochemistry
Periodontal ligament cells
Periodontal ligament cells

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Öhman, JennyLund University,Lunds universitet,Kärlfysiologi,Forskargrupper vid Lunds universitet,Vascular Physiology,Lund University Research Groups(Swepub:lu)mphy-jni (author)
Odenlund, MalinLund University,Lunds universitet,Kärlfysiologi,Forskargrupper vid Lunds universitet,Vascular Physiology,Lund University Research Groups(Swepub:lu)med-mod (author)
Bratthall, Gunilla (author)
Broman, JonasLund University,Lunds universitet,Neurofysiologi,Forskargrupper vid Lunds universitet,Neurophysiology,Lund University Research Groups(Swepub:lu)mphy-jbr (author)
Ekblad, EvaLund University,Lunds universitet,Neurogastroenterologi,Forskargrupper vid Lunds universitet,Neurogastroenterology,Lund University Research Groups(Swepub:lu)mphy-eek (author)
Lydrup, Marie-Louise (author)
Nilsson, Bengt-OlofLund University,Lunds universitet,Kärlfysiologi,Forskargrupper vid Lunds universitet,Vascular Physiology,Lund University Research Groups(Swepub:lu)mphy-bon (author)
Nilsson, Jenny (author)
KärlfysiologiForskargrupper vid Lunds universitet (creator_code:org_t)

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In:Archives of Oral Biology: Elsevier BV52:7, s. 669-6761879-15060003-9969

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By the author/editor: Jönsson, Daniel; Öhman, Jenny; Odenlund, Malin; Bratthall, Gunil ...; Broman, Jonas; Ekblad, Eva; show more...; Lydrup, Marie-Lo ...; Nilsson, Bengt-O ...; Nilsson, Jenny; show less...

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MEDICAL AND HEALTH SCIENCES: MEDICAL AND HEAL ...; and Basic Medicine; and Physiology

MEDICAL AND HEALTH SCIENCES: MEDICAL AND HEAL ...; and Clinical Medicin ...; and Hematology

Articles in the publication: Archives of Oral ...

By the university: Lund University; Malmö University

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