Demonstration of mitochondrial oestrogen receptor beta and oestrogen-induced attenuation of cytochrome c oxidase subunit I expression in human periodontal ligament cells.

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Fältnamn	Indikatorer	Metadata
000		05215naa a2200517 4500
001		oai:lup.lub.lu.se:3dd7cae9-8b7d-4c16-9917-266532ec4fa1
003		SwePub
008		160401s2007 \| \|\|\|\|\|\|\|\|\|\|\|000 \|\|eng\|
009		oai:DiVA.org:mau-15609
024	7	a https://lup.lub.lu.se/record/1649012 URI
024	7	a https://doi.org/10.1016/j.archoralbio.2006.12.0092 DOI
024	7	a https://urn.kb.se/resolve?urn=urn:nbn:se:mau:diva-156092 URI
040		a (SwePub)lud (SwePub)mau
041		a engb eng
042		9 SwePub
072	7	a art2 swepub-publicationtype
072	7	a ref2 swepub-contenttype
100	1	a Jönsson, Danielu Malmö högskola,Lund University,Lunds universitet,Kärlfysiologi,Forskargrupper vid Lunds universitet,Vascular Physiology,Lund University Research Groups,Odontologiska fakulteten (OD)4 aut0 (Swepub:mau)oddajo
245	1 0	a Demonstration of mitochondrial oestrogen receptor beta and oestrogen-induced attenuation of cytochrome c oxidase subunit I expression in human periodontal ligament cells.
264	1	b Elsevier BV,c 2007
520		a OBJECTIVE: Periodontal ligament (PDL) cells express oestrogen receptor beta (ERbeta) protein, but cellular functions regulated by ERbeta in these cells have not been identified. In this study we determine if ERbeta is localised to mitochondria and if oestrogen regulates mitochondrial function in human PDL cells obtained from teeth extracted for orthodontic reasons. DESIGN: Subcellular distribution of ERbeta was determined by confocal microscopy of cells co-stained with ERbeta antibody and the mitochondrion-selective probe MitoTracker and by immunogold electron microscopy. Expression of the mitochondrial enzyme cytochrome c oxidase subunit I, involved in oxidative phosphorylation, was determined by Western blotting in cells treated with or without physiological concentrations of the endogenous oestrogen 17beta-oestradiol. RESULTS: ERbeta immunoreactivity was observed both in the nuclei and the cytoplasm. MitoTracker-labelling was observed in the cytoplasm, especially in the perinuclear region, but not in the nuclei. Co-localisation of ERbeta and MitoTracker was observed in cells derived from both male and female subjects. Mitochondrial localisation of ERbeta was confirmed by immunogold electron microscopy. Cells treated with or without 17beta-oestradiol (100 nM) displayed an identical pattern of staining for mitochondria. Treatment with 100 nM 17beta-oestradiol attenuated cytochrome c oxidase subunit I expression by about 30%, while combined treatment with 17beta-oestradiol and the ER blocker ICI 182780 (10 microM) had no effect. CONCLUSION: This study demonstrates mitochondrial localisation of ERbeta and oestrogen-induced decrease in the expression of cytochrome c oxidase subunit I in human PDL cells, suggesting that oestrogen probably via ERbeta influences mitochondrial function and PDL cell energy
650	7	a MEDICIN OCH HÄLSOVETENSKAPx Medicinska och farmaceutiska grundvetenskaperx Fysiologi0 (SwePub)301062 hsv//swe
650	7	a MEDICAL AND HEALTH SCIENCESx Basic Medicinex Physiology0 (SwePub)301062 hsv//eng
650	7	a MEDICIN OCH HÄLSOVETENSKAPx Klinisk medicinx Hematologi0 (SwePub)302022 hsv//swe
650	7	a MEDICAL AND HEALTH SCIENCESx Clinical Medicinex Hematology0 (SwePub)302022 hsv//eng
653		a Oxidative phosphorylation
653		a Mitochondria
653		a Oestrogen
653		a Immunocytochemistry
653		a Periodontal ligament cells
653		a Periodontal ligament cells
700	1	a Öhman, Jennyu Lund University,Lunds universitet,Kärlfysiologi,Forskargrupper vid Lunds universitet,Vascular Physiology,Lund University Research Groups4 aut0 (Swepub:lu)mphy-jni
700	1	a Odenlund, Malinu Lund University,Lunds universitet,Kärlfysiologi,Forskargrupper vid Lunds universitet,Vascular Physiology,Lund University Research Groups4 aut0 (Swepub:lu)med-mod
700	1	a Bratthall, Gunilla4 aut
700	1	a Broman, Jonasu Lund University,Lunds universitet,Neurofysiologi,Forskargrupper vid Lunds universitet,Neurophysiology,Lund University Research Groups4 aut0 (Swepub:lu)mphy-jbr
700	1	a Ekblad, Evau Lund University,Lunds universitet,Neurogastroenterologi,Forskargrupper vid Lunds universitet,Neurogastroenterology,Lund University Research Groups4 aut0 (Swepub:lu)mphy-eek
700	1	a Lydrup, Marie-Louise4 aut
700	1	a Nilsson, Bengt-Olofu Lund University,Lunds universitet,Kärlfysiologi,Forskargrupper vid Lunds universitet,Vascular Physiology,Lund University Research Groups4 aut0 (Swepub:lu)mphy-bon
700	1	a Nilsson, Jenny4 aut
710	2	a Kärlfysiologib Forskargrupper vid Lunds universitet4 org
773	0	t Archives of Oral Biologyd : Elsevier BVg 52:7, s. 669-676q 52:7<669-676x 1879-1506x 0003-9969
856	4	u http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=17223066&dopt=Abstracty FULLTEXT
856	4	u http://dx.doi.org/10.1016/j.archoralbio.2006.12.009y FULLTEXT
856	4 8	u https://lup.lub.lu.se/record/164901
856	4 8	u https://doi.org/10.1016/j.archoralbio.2006.12.009
856	4 8	u https://urn.kb.se/resolve?urn=urn:nbn:se:mau:diva-15609

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By the author/editor: Jönsson, Daniel; Öhman, Jenny; Odenlund, Malin; Bratthall, Gunil ...; Broman, Jonas; Ekblad, Eva; show more...; Lydrup, Marie-Lo ...; Nilsson, Bengt-O ...; Nilsson, Jenny; show less...

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MEDICAL AND HEALTH SCIENCES: MEDICAL AND HEAL ...; and Basic Medicine; and Physiology

MEDICAL AND HEALTH SCIENCES: MEDICAL AND HEAL ...; and Clinical Medicin ...; and Hematology

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By the university: Lund University; Malmö University

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