WFRF:(Park James)
Search: WFRF:(Park James) > (2005-2009) > Characterization of...
- 9 of 9
- Previous record
- Next record
- To hitlist
Characterization of Global Yeast Quantitative Proteome Data Generated from the Wild-Type and Glucose Repression Saccharomyces cerevisiae Strains: The comparison of two Quantitative Methods
- Usaite, Renata (author)
- Wohlschlegel, James (author)
- Venable, John D. (author)
- show more...
- Park, Sung K. (author)
- Nielsen, Jens B, 1962 (author)
- Olsson, Lisbeth, 1963 (author)
- Yates, John R. (author)
- show less...
- 2008
- 2008
- English.
- In: Journal of Proteome Research. - 1535-3907 .- 1535-3893. ; 266:7, s. 266-275
- Journal article (peer-reviewed)
Abstract
Subject headings
Close
- The quantitative proteomic analysis of complex protein mixtures is emerging as a technically challenging but viable systems-level approach for studying cellular function. This study presents a large-scale comparative analysis of protein abundances from yeast protein lysates derived from both wild-type yeast and yeast strains lacking key components of the Snf1 kinase complex. Four different strains were grown under well-controlled chemostat conditions. Multidimensional protein identification technology followed by quantitation using either spectral counting or stable isotope labeling approaches was used to identify relative changes in the protein expression levels between the strains. A total of 2388 proteins were relatively quantified, and more than 350 proteins were found to have significantly different expression levels between the two strains of comparison when using the stable isotope labeling strategy. The stable isotope labeling based quantitative approach was found to be highly reproducible among biological replicates when complex protein mixtures containing small expression changes were analyzed. Where poor correlation between stable isotope labeling and spectral counting was found, the major reason behind the discrepancy was the lack of reproducible sampling for proteins with low spectral counts. The functional categorization of the relative protein expression differences that occur in Snf1-deficient strains uncovers a wide range of biological processes regulated by this important cellular kinase.
Subject headings
- TEKNIK OCH TEKNOLOGIER -- Industriell bioteknik (hsv//swe)
- ENGINEERING AND TECHNOLOGY -- Industrial Biotechnology (hsv//eng)
Keyword
- proteomics
- AMP-activated kinase
- CenSus
- MudPIT
- 15N
- Snf4
- mass spectrometry
Publication and Content Type
- art (subject category)
- ref (subject category)
Find in a library
- Journal of Proteome Research (Search for host publication in LIBRIS)
To the university's database
- 9 of 9
- Previous record
- Next record
- To hitlist
Find more in SwePub
- By the author/editor
- Usaite, Renata
- Wohlschlegel, Ja ...
- Venable, John D.
- Park, Sung K.
- Nielsen, Jens B, ...
- Olsson, Lisbeth, ...
- show more...
- Yates, John R.
- show less...
- About the subject
-
- ENGINEERING AND TECHNOLOGY
- ENGINEERING AND ...
- and Industrial Biote ...
- Articles in the publication
- Journal of Prote ...
- By the university
- Chalmers University of Technology
Search outside SwePub
- Extend your search to:
- Google Book Search
- Google Scholar
Kungliga biblioteket hanterar dina personuppgifter i enlighet med EU:s dataskyddsförordning (2018), GDPR. Läs mer om hur det funkar här.
Så här hanterar KB dina uppgifter vid användning av denna tjänst.
- Copyright © LIBRIS - National Library Systems
- LIBRIS.kb.se
